Detailed information of TA system TA179316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67128..67864
• Replicon: plasmid pBS419-3
• Accession: NZ_CP063903
• Organism: Klebsiella quasipneumoniae strain BS419-3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ISA85_RS26185
• Protein ID: WP_003026803.1
• Coordinates: 67382..67864 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA85_RS26180
• Protein ID: WP_003026799.1
• Coordinates: 67128..67394 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA85_RS26135	63190..63552	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
ISA85_RS26140	63602..63952	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ISA85_RS26145	64310..64579	+	270	WP_019706036.1	hypothetical protein	-
ISA85_RS26150	64567..65142	+	576	WP_004152103.1	hypothetical protein	-
ISA85_RS26155	65173..65667	+	495	WP_004152104.1	DNA-binding protein	-
ISA85_RS26160	65711..66079	+	369	WP_004152105.1	hypothetical protein	-
ISA85_RS26165	66113..66316	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
ISA85_RS26170	66365..66622	+	258	WP_004152107.1	hypothetical protein	-
ISA85_RS26175	66698..66952	+	255	WP_004152108.1	hypothetical protein	-
ISA85_RS26180	67128..67394	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA85_RS26185	67382..67864	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ISA85_RS26190	68065..69468	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
ISA85_RS26195	69497..70129	-	633	WP_001567369.1	hypothetical protein	-
ISA85_RS26200	70306..71560	-	1255	Protein_67	IS3 family transposase	-
ISA85_RS26205	71648..72610	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..163712	163712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T179316 WP_003026803.1 NZ_CP063903:67382-67864 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179316 NZ_CP084683:c4116972-4116574 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179316 WP_003026799.1 NZ_CP063903:67128-67394 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179316 NZ_CP084683:c4117268-4116975 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66