Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1535992..1536611 Replicon chromosome
Accession NZ_CP063902
Organism Klebsiella quasipneumoniae strain BS419-3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA85_RS07515 Protein ID WP_002892050.1
Coordinates 1536393..1536611 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA85_RS07510 Protein ID WP_002892066.1
Coordinates 1535992..1536366 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA85_RS07500 1531145..1532338 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISA85_RS07505 1532361..1535507 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit -
ISA85_RS07510 1535992..1536366 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA85_RS07515 1536393..1536611 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA85_RS07520 1536770..1537336 + 567 WP_004204750.1 maltose O-acetyltransferase -
ISA85_RS07525 1537308..1537430 - 123 WP_032426076.1 hypothetical protein -
ISA85_RS07530 1537473..1537943 + 471 WP_004204751.1 YlaC family protein -
ISA85_RS07535 1537912..1539369 - 1458 WP_064178013.1 PLP-dependent aminotransferase family protein -
ISA85_RS07540 1539470..1540168 + 699 WP_004204753.1 GNAT family N-acetyltransferase -
ISA85_RS07545 1540165..1540305 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA85_RS07550 1540305..1540568 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179305 WP_002892050.1 NZ_CP063902:1536393-1536611 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179305 NZ_CP084683:c239095-238781 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179305 WP_002892066.1 NZ_CP063902:1535992-1536366 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179305 NZ_CP084683:c238784-238368 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References