Detailed information of TA system TA179302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 113963..114873
• Replicon: plasmid pBS324-2
• Accession: NZ_CP063901
• Organism: Klebsiella quasipneumoniae strain BS324-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ISA86_RS25345
• Protein ID: WP_032413375.1
• Coordinates: 113963..114433 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: ISA86_RS25350
• Protein ID: WP_032413443.1
• Coordinates: 114430..114873 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA86_RS25325	110906..112159	+	1254	WP_194417906.1	lactose permease	-
ISA86_RS25330	112225..112287	+	63	Protein_113	galactoside O-acetyltransferase	-
ISA86_RS25335	112319..113287	+	969	WP_074422432.1	IS5-like element IS903B family transposase	-
ISA86_RS25340	113593..113853	+	261	WP_017901409.1	hypothetical protein	-
ISA86_RS25345	113963..114433	-	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
ISA86_RS25350	114430..114873	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
ISA86_RS25355	114973..115485	-	513	Protein_118	DUF4113 domain-containing protein	-
ISA86_RS25360	115586..115771	-	186	WP_071571073.1	DUF2188 domain-containing protein	-
ISA86_RS25365	115791..116009	+	219	Protein_120	transposase	-
ISA86_RS25370	116290..116517	-	228	Protein_121	transposase	-
ISA86_RS25375	116575..116922	-	348	WP_032413377.1	hypothetical protein	-
ISA86_RS25380	117059..118237	-	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
ISA86_RS25385	118284..119282	-	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..176438	176438
	-	inside	IScluster/Tn	-	-	112319..116547	4228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T179302 WP_032413375.1 NZ_CP063901:c114433-113963 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T179302 NZ_CP084664:3764493-3764672 [Hafnia paralvei]
ATGAATAGCGGGATGCTGATCGCTGAAATCATGGCTGACGGGTGGATCCTTAAAAGGGTGAAGGGAAGCCATCATCACTT
CACTCACCCCAACAAAACGGGGCTAGTGACGATTCCTCATCTTAAAAAAGATCTCCCCGCCAATACCGTAAAAAGCATCC
GGCAACAGGCTGGGCTTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT179302 WP_032413443.1 NZ_CP063901:c114873-114430 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT179302 NZ_CP084664:3764695-3765105 [Hafnia paralvei]
ATGCTTTATCCCATTGCTATTGAATCCGGAGATCAACACCATTCTTACGGTGTAACCGTGCCTGATTTACCGGGGTGTTT
CTCTGCCGGTGATACGCTCGATGAAGCAATAGCTAACGCCAAGCAAGCAATCATTAGTCATATTGAATTGCTGGTTGAAA
TGGAAGCCGATTTTCCAATGCCATCAAAAATCGAAGATTTATCAAACAAAGCTGAATATCAGGAATACATCTGGGCTATC
GTCGAGGTTGATGTTACTCGCCTCATGGGCGGCGCAGAAAAGATTAACGTCACGCTCCCTAAGTCATTGATTGCCAGAAT
CGATCGCGTCGTTGCCTTACATCCTGAATATAAAAGCCGATCCGGTTTTTTAGCTCAGGCTGCGCTCGAACAGGTAACGG
TGATAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4