Detailed information of TA system TA179301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 58496..59232
• Replicon: plasmid pBS324-2
• Accession: NZ_CP063901
• Organism: Klebsiella quasipneumoniae strain BS324-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: ISA86_RS25055
• Protein ID: WP_004187044.1
• Coordinates: 58496..58978 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA86_RS25060
• Protein ID: WP_003026799.1
• Coordinates: 58966..59232 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA86_RS25035	54891..55004	-	114	WP_077253754.1	IS3 family transposase	-
ISA86_RS25040	55425..55538	-	114	Protein_55	transposase	-
ISA86_RS25045	56479..57255	-	777	Protein_56	DUF1173 family protein	-
ISA86_RS25050	57288..58256	+	969	WP_194417912.1	IS5 family transposase	-
ISA86_RS25055	58496..58978	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
ISA86_RS25060	58966..59232	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA86_RS25065	60344..61153	-	810	WP_135716166.1	N-formylglutamate deformylase	-
ISA86_RS25070	61146..62354	-	1209	WP_194417913.1	imidazolonepropionase	-
ISA86_RS25075	62366..62887	-	522	Protein_62	cytosine permease	-
ISA86_RS25080	62944..63912	-	969	WP_004099053.1	IS5-like element IS903B family transposase	-
ISA86_RS25085	64020..64223	-	204	WP_004150739.1	hemolysin expression modulator Hha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	57288..63912	6624
	-	inside	Non-Mobilizable plasmid	-	-	1..176438	176438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T179301 WP_004187044.1 NZ_CP063901:c58978-58496 [Klebsiella quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T179301 NZ_CP084664:3108266-3108469 [Hafnia paralvei]
ATGACTAAAATTGATTACCTGATGCGTTTGCGCAAGTGCACTACCTTAGACACTCTCGAACGCGTGATTGAAAAAAATAA
GTATGAACTTTCCGACGATGAGCTAGAGACCTTCTACTCTGCGGCAGATCACCGTCTGGCAGAGCTAACGATGAACAAGC
TATATGACAAGATCCCAGCGGAAGTGTGGAAGTATGTACGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179301 WP_003026799.1 NZ_CP063901:c59232-58966 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179301 NZ_CP084664:3107878-3108252 [Hafnia paralvei]
ATGGATGAATATACGTCTTATCAGCACGATATTACGGAGCTGAAATATCTATGTAATATGTTGTACAATCAAGGAATGGA
TGTTCTGAGCGATAGCCATCATGGCTGGGTCAACGACCCAACAGCAGTGGACAATCTACAACTAAATGAATTAAACGAAC
ATATTGCAAATTTTGGTTTAACATTCAAGATTAAGTATCCAAATGCTTCAGAATTAACTGAAATATTAGATGAGTATCTT
GACGAAACATACAGTCTGTTTAGTAATTACAGCATCAACGAACCAGAACTTAAAAAATGGCTTAAAGCAAAAGGCCGTAT
TTTGCGTTATTTAGCGGGTGAAAAAACCACATCTGCATTAAATTTTAACCTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66