Detailed information of TA system TA179295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1549066..1550858
• Replicon: chromosome
• Accession: NZ_CP063900
• Organism: Klebsiella quasipneumoniae strain BS324-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISA86_RS07730
• Protein ID: WP_109938479.1
• Coordinates: 1549066..1550415 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: ISA86_RS07735
• Protein ID: WP_064178387.1
• Coordinates: 1550412..1550858 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA86_RS07705	1544482..1545414	-	933	WP_004201619.1	tRNA dihydrouridine(16) synthase DusC	-
ISA86_RS07710	1545772..1546176	+	405	WP_004201620.1	CidA/LrgA family protein	-
ISA86_RS07715	1546166..1546861	+	696	WP_004201621.1	CidB/LrgB family autolysis modulator	-
ISA86_RS07720	1546990..1547874	+	885	WP_049093651.1	cytidine deaminase	-
ISA86_RS07725	1548220..1548951	+	732	WP_008804033.1	outer membrane permeability protein SanA	-
ISA86_RS07730	1549066..1550415	+	1350	WP_109938479.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA86_RS07735	1550412..1550858	+	447	WP_064178387.1	helix-turn-helix domain-containing protein	Antitoxin
ISA86_RS07740	1550900..1551910	-	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
ISA86_RS07745	1551926..1553446	-	1521	WP_004201625.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
ISA86_RS07750	1553565..1554563	-	999	WP_004201626.1	galactose/glucose ABC transporter substrate-binding protein MglB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-410)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51080.97 Da
• Isoelectric Point: 4.8038

>T179295 WP_109938479.1 NZ_CP063900:1549066-1550415 [Klebsiella quasipneumoniae]
METLTVQAFLNAEWQDIALIKFPGSEQGDWFTTQIDYLTDYAIDFLERDDYHAVSLNHPVSLYFEDHGNPGWLRFIDDII
PSGASRRYWVNFLDISELPQGQQNFILLKFGTMSPVGNLRIKDSVPDWDSLASSKTFSVTDVMNRASDFLDYAQERGAAA
GGATGAGGEAPKLLLRCSKRQEIWIDTWQNEPGSDDLYYLVKYPRGSRSEVDCNILRAEFHYYHELTTMGFSTIPVDNMR
LEEGLHYPSLWLPRFDIRRDGDGRIMRLGMESVYSLLQKAPATILDHETTLRILIEKITSSHMVRVQNYRFDVQGFIIDW
VRRDLLNIIFGNSDNHGRNTAFMKADNEITLAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWIAPDT
LLNALRETASQLIDLPSRLKARGVPVQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 1350 bp

>T179295 NZ_CP084656:3738438-3738620 [Pectobacterium versatile]
ATGACATCAACTGACCTGATAAAGGAGCTGGCAGCGGCAGGTTGTGAGTGCAAACGGCATAACGGCGGGAGTCACCAAAT
ATGGTGGTCACCCATTACTGGAAAAACATTTCCAGTGCCGCATCCAAAAAAGGATTTACCACTTGGAACTGTCAAATCCA
TCAAAAAAATGGCGGGGATCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16510.20 Da
• Isoelectric Point: 10.5137

>AT179295 WP_064178387.1 NZ_CP063900:1550412-1550858 [Klebsiella quasipneumoniae]
MKTLNKKETEIQNTLARLRAENPPSLPATEVTATEKKARSEISRQRVSTGLKKVKTIDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIFKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT179295 NZ_CP084656:3738643-3739074 [Pectobacterium versatile]
ATGTTTTTTTCAGTCGGTGTTGAAACGCCAAAAGATGATGCTACGGCATATGGGCTGATCGTTCCGGTGTTGTGTACTGA
TGATTATGGCTGCTTCTCTGCAGCTGACAGCCAACAAGAGATTGCACGCATGGCGCGGGAAGCGATTTTAACCATTATTG
ATGAAGCGGTGAGCCGCGGAAATCTGGATATTAAACGACTTAATGATGCTGGACATCTCATCTATGCGGCAATACCAGAT
TTTGCGGACTACGATAGTTGGTTCGTGCTGGATGTGGATTTATCGGCTTTTGAGGGAAAACCGCAGCGATTGAACATTTC
ACTTCCCGATACGTTGATACAGCGTATTGATAATCGCGTGCGAGAGCAGCCGGGTACTTATCGCGATCGCAGCCATTTTT
TGGCAGAAGCCGCACGCCATGAACTGGAGTGA