Detailed information of TA system TA179286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 563400..564988
• Replicon: chromosome
• Accession: NZ_CP063900
• Organism: Klebsiella quasipneumoniae strain BS324-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISA86_RS02845
• Protein ID: WP_004205919.1
• Coordinates: 563666..564988 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: ISA86_RS02840
• Protein ID: WP_004205921.1
• Coordinates: 563400..563666 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA86_RS02815	558533..559456	+	924	WP_194417686.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA86_RS02820	559437..560591	-	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
ISA86_RS02825	560937..562316	+	1380	WP_194417687.1	purine permease	-
ISA86_RS02830	562294..562794	+	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA86_RS02835	562791..563117	+	327	WP_069602708.1	hydroxyisourate hydrolase	-
ISA86_RS02840	563400..563666	+	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA86_RS02845	563666..564988	+	1323	WP_004205919.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA86_RS02850	565005..566297	-	1293	WP_049093368.1	glycoside hydrolase family 10 protein	-
ISA86_RS02855	566439..566645	-	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
ISA86_RS02860	566648..567181	-	534	WP_004205916.1	hypothetical protein	-
ISA86_RS02865	567210..567554	-	345	WP_004205915.1	hypothetical protein	-
ISA86_RS02870	567541..568656	-	1116	WP_194417688.1	serine hydrolase	-
ISA86_RS02875	568925..569707	-	783	WP_049003659.1	type I methionyl aminopeptidase	-
ISA86_RS02880	569688..569906	-	219	WP_002904808.1	ParD-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaOKP-B-15	iroE / mgtC	360918..725416	364498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49013.27 Da
• Isoelectric Point: 8.2655

>T179286 WP_004205919.1 NZ_CP063900:563666-564988 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Nucleotide

• Length: 1323 bp

>T179286 NZ_CP084656:2909189-2909569 [Pectobacterium versatile]
ATGCAAACCTTACCTGTTTCACCAGAACGGGCGGCTCGCGCCTGCCCGTCACCAGTCGAAGTCTGGCAAATGCTGCTGAC
CCATCTGCTGTCACAGCATTATGGCCTGGCACTGAACGACACCCCATTTAGCGACGACAATATCATTCAGGAGCATATTG
ATGCCGGTATATCCCTGTGTGATGCCGTCAATTTTATCGTCGAAAAGTATGAACTGGTGCGCACCGACCGCCCGGGATTC
AGTGTCACGGAACAGTCAGCACTCATCACCCGTATCGATATCCTTCGTGCCAGGAAGGCCTGCGGACTGATGACCCGCAA
CGGCTACAAAGCCGTGACAAACATCACCTGTGGCAACAAACCGCAGGAGCCCGGACGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT179286 WP_004205921.1 NZ_CP063900:563400-563666 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT179286 NZ_CP084656:2908758-2909126 [Pectobacterium versatile]
TTGTCGCAAACAACACCAGCTACACCCGCCGTAAATCATGACGTCACAGAGCCCAGGTGGGGGCTGAAGCGCGATATCAC
CCCGTGCTTTGGCTCACGTCTGGTGCAGGAAGGCAACCGTCTGCACTACCTGGCTGACCGCGCCAGTATCACCGGTATAT
TCAGTAATGAAGATTTGCGTCACCTCGACCAGGCCTTCCCGCTGCTGCTGAAACAGTTAGAACTAATGCTGAGCAGCGGG
GTGCTAAATCCCCGCCACCCGCACTGTGTCACGCTGCATGCGAAAGGATTAACCTGCGACGCTGACACGCGGGGTAGTTG
CGGTTATCTGTATATTTCGATTTATCCCACCCCCACGGCAAACGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9