Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 76927..77666 Replicon plasmid pBS326-1
Accession NZ_CP063899
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag ISA87_RS26805 Protein ID WP_013087172.1
Coordinates 77181..77666 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag ISA87_RS26800 Protein ID WP_012540086.1
Coordinates 76927..77193 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS26770 72158..72694 - 537 WP_013087166.1 GNAT family N-acetyltransferase -
ISA87_RS26775 72819..74570 - 1752 WP_162875198.1 arsenical pump-driving ATPase -
ISA87_RS26780 74597..74959 - 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
ISA87_RS26785 75035..75580 - 546 WP_153669792.1 sigma-70 family RNA polymerase sigma factor -
ISA87_RS26790 75589..76302 - 714 WP_013087170.1 arsenical resistance protein ArsH -
ISA87_RS26795 76304..76627 - 324 WP_153669794.1 metalloregulator ArsR/SmtB family transcription factor -
ISA87_RS26800 76927..77193 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
ISA87_RS26805 77181..77666 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
ISA87_RS26810 78008..78166 + 159 WP_014386184.1 type I toxin-antitoxin system Hok family toxin -
ISA87_RS26815 79070..79450 + 381 WP_038990973.1 hypothetical protein -
ISA87_RS26820 79517..79864 + 348 WP_038990975.1 hypothetical protein -
ISA87_RS26825 79952..80182 + 231 WP_038990977.1 hypothetical protein -
ISA87_RS26830 80229..81062 + 834 WP_040107920.1 type I restriction-modification system subunit M -
ISA87_RS26835 81687..82217 + 531 WP_040107921.1 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139497 139497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T179285 WP_013087172.1 NZ_CP063899:77181-77666 [Klebsiella variicola]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T179285 NZ_CP084656:2886837-2887121 [Pectobacterium versatile]
ATGAGTTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGGGTGCGCCCGTGCGGGAGCAGTTCAC
CAAAAAGTTGAAAGAGGTTTTACAAAATCCCCACGTTCCCGCTAACCGACTTCATGGAATGGCTGACTGCTACAAAATCA
AACTTCGTTCCGCTGGCTATCGGTTAGTGTATCAAGTACTTGAGCATGAGATCGTGGTGCTGGTTTTAGCTGTGGGCAAA
CGGGAACGCTCAGAGGTTTATAAAGCAGCGAAAGACCGACTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT179285 WP_012540086.1 NZ_CP063899:76927-77193 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT179285 NZ_CP084656:2886596-2886847 [Pectobacterium versatile]
ATGTCCGTACAACCTATCCTTGCAAATGCAGCCGTGAGTATCAGCGACTTTAAAAAGTCCCCTAATGCCGCGCTTAAAGA
AGCTAACGGCGAGCCTGTAGCCGTGCTAACCAATGGCCGTATCTCTGGCTATTATGTTTCTCCTGAAACATGGGAAGCAC
TGGCCGATTATCAGGAAGATATCGAACTCGCCAAAATTGCCCGTTCCCGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References