Detailed information of TA system TA179284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 28654..29390
• Replicon: plasmid pBS326-1
• Accession: NZ_CP063899
• Organism: Klebsiella variicola strain BS326-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ISA87_RS26525
• Protein ID: WP_194389026.1
• Coordinates: 28654..29136 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA87_RS26530
• Protein ID: WP_003026799.1
• Coordinates: 29124..29390 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA87_RS26510	24850..25851	-	1002	WP_040107896.1	LacI family transcriptional regulator	-
ISA87_RS26515	26433..26852	-	420	WP_012540023.1	ASCH domain-containing protein	-
ISA87_RS26520	27040..28440	-	1401	WP_153669771.1	ISNCY family transposase	-
ISA87_RS26525	28654..29136	-	483	WP_194389026.1	GNAT family N-acetyltransferase	Toxin
ISA87_RS26530	29124..29390	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA87_RS26535	29515..29820	-	306	WP_194389033.1	hypothetical protein	-
ISA87_RS26540	29830..30036	-	207	Protein_25	hypothetical protein	-
ISA87_RS26545	30100..30330	-	231	WP_062913614.1	hypothetical protein	-
ISA87_RS26550	30344..30547	-	204	WP_012540085.1	hemolysin expression modulator Hha	-
ISA87_RS26555	30597..31091	-	495	WP_087661550.1	DNA-binding protein	-
ISA87_RS26560	31122..31688	-	567	WP_087661549.1	hypothetical protein	-
ISA87_RS26565	31685..31948	-	264	WP_087661548.1	hypothetical protein	-
ISA87_RS26570	32278..33381	+	1104	WP_087661547.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..139497	139497
	-	flank	IS/Tn	-	-	27040..28440	1400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17384.00 Da
• Isoelectric Point: 9.5822

>T179284 WP_194389026.1 NZ_CP063899:c29136-28654 [Klebsiella variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKTSQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179284 NZ_CP084656:c2886043-2885564 [Pectobacterium versatile]
GTGGGAATAACGGCACCTGAATTATTATTACCTCAGCATGCCGTGGTTGATTTCCGAAGTTCAGAACCGTCACTGGATGA
TTGGCTGAAACGTAAGGCGCTCAAGAATCAAACCATTGGCGCATCACGCACTTTTGTCGTGTGTGAATCGGGTGCTAATC
AGGTTATTGGCTTTTACGCGCTGGCAACCGGCAGCGTACAGCGTCAATCCGTATCGGGTGCGTTACGACGCAATATGCCT
GATCCGCTGCCCGTATTAGTCTTGGGCCGTCTGGCGGTAGATGAACGTTATCACCGTCGGGGGATTGGCGCGGGACTATT
GAAAGATGCGGTCTTGCGTTCCCAGCAGGTTGCTGAACAGGTGGGAACAAAAGCGTTATTAGTTCATGCGCTGTCTGAGG
AAGCAAAAGATTTTTATCTGCATTGGGGCTTTACGTCCTCGGAAATCCAGCCTAGCACATTGCTTCTACCACTCTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179284 WP_003026799.1 NZ_CP063899:c29390-29124 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179284 NZ_CP084656:c2886306-2886034 [Pectobacterium versatile]
ATGAGAACAGAGGCAAAAGAGGCACCGATCAACATTCGGGCGAAATCATCACAACGGGAACTGATAGACGTTGCCGCGAA
ACTGCTTTCAAAGTCACGTACTGATTTTATCCTTGAGGCTGCATGTCGTGAGGCGGAAGAGGTGCTATTGGATCAACGCC
TGTTTCTGGTTGATGAATCACAATTTGATGCCTTTATTACCGCGTTAGAAAAACCTGTCGCAACCAATGAGCGGTTACAG
GCGTTATTAGACAGGAAATCACCGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66