Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 5425731..5426317 Replicon chromosome
Accession NZ_CP063898
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name doc Uniprot ID A0A2W7TCK5
Locus tag ISA87_RS26175 Protein ID WP_008806973.1
Coordinates 5425731..5426099 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISA87_RS26180 Protein ID WP_004174006.1
Coordinates 5426096..5426317 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS26155 5421220..5422290 - 1071 WP_023323353.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
ISA87_RS26160 5422292..5423137 - 846 WP_008806976.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA87_RS26165 5423134..5424021 - 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA87_RS26170 5424123..5425439 - 1317 WP_008806974.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA87_RS26175 5425731..5426099 - 369 WP_008806973.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA87_RS26180 5426096..5426317 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA87_RS26185 5426481..5427194 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA87_RS26190 5427196..5427963 - 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA87_RS26195 5427960..5429237 - 1278 WP_194388799.1 branched chain amino acid ABC transporter permease LivM -
ISA87_RS26200 5429234..5430160 - 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5420483..5440961 20478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13639.01 Da        Isoelectric Point: 7.3191

>T179282 WP_008806973.1 NZ_CP063898:c5426099-5425731 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179282 NZ_CP084656:2778697-2778984 [Pectobacterium versatile]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCCGGGAGCAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAACTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGAGAAGGTAGCCAGATTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179282 WP_004174006.1 NZ_CP063898:c5426317-5426096 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179282 NZ_CP084656:2778453-2778707 [Pectobacterium versatile]
ATGGCTTTCAACATCCTGACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTACGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGCGTGCCTCCTGCCGTATTCGCTTATTTAA
TGGAACTGGCTGAAGATGCCGAACTGGGGCGTATCGTTGATGACCGGATGTCAGAACTAGCTGACGCGGAAGAGATTTCG
CTTGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W7TCK5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References