Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3980940..3981530 Replicon chromosome
Accession NZ_CP063898
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA87_RS19190 Protein ID WP_008804165.1
Coordinates 3980940..3981272 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA87_RS19195 Protein ID WP_012541132.1
Coordinates 3981273..3981530 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS19165 3976978..3977223 + 246 WP_008804171.1 signal transduction protein PmrD -
ISA87_RS19170 3977354..3978808 + 1455 WP_064141536.1 AMP nucleosidase -
ISA87_RS19180 3979181..3980617 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA87_RS19190 3980940..3981272 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA87_RS19195 3981273..3981530 - 258 WP_012541132.1 antitoxin Antitoxin
ISA87_RS19200 3982057..3983157 + 1101 WP_048271881.1 phosphotransferase -
ISA87_RS19205 3983154..3984125 - 972 WP_048271882.1 sensor domain-containing diguanylate cyclase -
ISA87_RS19210 3984332..3985168 - 837 WP_048271883.1 alpha/beta hydrolase -
ISA87_RS19215 3985345..3985920 - 576 WP_048271884.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179280 WP_008804165.1 NZ_CP063898:c3981272-3980940 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179280 NZ_CP063898:c3981272-3980940 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179280 WP_012541132.1 NZ_CP063898:c3981530-3981273 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179280 NZ_CP063898:c3981530-3981273 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References