Detailed information of TA system TA179271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3080058..3081646
• Replicon: chromosome
• Accession: NZ_CP063898
• Organism: Klebsiella variicola strain BS326-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0B7G636
• Locus tag: ISA87_RS14695
• Protein ID: WP_012968244.1
• Coordinates: 3080324..3081646 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISA87_RS14690
• Protein ID: WP_008804920.1
• Coordinates: 3080058..3080324 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA87_RS14665	3075199..3076122	+	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA87_RS14670	3076103..3077257	-	1155	WP_117115537.1	FAD-dependent urate hydroxylase HpxO	-
ISA87_RS14675	3077603..3078982	+	1380	WP_012968246.1	purine permease	-
ISA87_RS14680	3078960..3079460	+	501	WP_064170224.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA87_RS14685	3079457..3079792	+	336	WP_088717111.1	hydroxyisourate hydrolase	-
ISA87_RS14690	3080058..3080324	+	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA87_RS14695	3080324..3081646	+	1323	WP_012968244.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA87_RS14700	3081663..3082955	-	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISA87_RS14705	3083087..3084091	-	1005	WP_088717112.1	type 1 fimbrial protein	-
ISA87_RS14710	3084079..3086580	-	2502	WP_049153029.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48896.29 Da
• Isoelectric Point: 7.9109

>T179271 WP_012968244.1 NZ_CP063898:3080324-3081646 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGELMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179271 NZ_CP084655:3560928-3561104 [Pectobacterium brasiliense]
GTGAAGCAAAGCGAGTTCAGGCGGTGGCTTGAATCTCAGGGGGTCGAAGTTTCAAACGGTACAAATCATTTGAAGCTGAG
GTACAACGGGAACCGAAGCGTAATGCCGAGACACCCGAGCGCTGAGATAAAAGAACCATTGCGAAAGGCCATACTTAAAC
AGCTTGGCCTGAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179271 WP_008804920.1 NZ_CP063898:3080058-3080324 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179271 NZ_CP084655:3561153-3561560 [Pectobacterium brasiliense]
ATGCGATATCCAGTAAATTTAGACCCGGACTCAGGCGGCTATGTTGTGTCGTTCCCTGATATACCGGAAGCCCTGACGCA
AGGGGATACGCGAGAGGATGCGTTAAAAGAGGCGCTGGATGCGCTGGTTACAGCCTTCGAGTTTTATTTCGAGGATAACC
AGCGTATTCCGGCACCAGGTAACATAACGGGTGATTGCGTAGAGGTTCCGGCCAGCATTGCTGCTAAGGTTTTAATGCTG
AATGCGTTTGTTGATTCCGGCTTAACTCAGGTTGAGTTGGCGTCACGCATGGGTGTAAAAAAACAGGAAGTAACCCGACT
GTTTGACCTGCATCACTCAACGAAAATAGACACAATTCAAAAGGCGATCTCTGCAATGGGATTGCGCCTGGAGCTGGTGG
CCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7G636

Antitoxin

• AlphaFold DB: A0A1F2MCN9