Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1557176..1557795 Replicon chromosome
Accession NZ_CP063898
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA87_RS07340 Protein ID WP_002892050.1
Coordinates 1557176..1557394 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA87_RS07345 Protein ID WP_008805436.1
Coordinates 1557421..1557795 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS07305 1553220..1553483 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA87_RS07310 1553483..1553623 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA87_RS07315 1553620..1554318 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA87_RS07320 1554419..1555876 + 1458 WP_117115360.1 PLP-dependent aminotransferase family protein -
ISA87_RS07325 1555845..1556315 - 471 WP_008805434.1 YlaC family protein -
ISA87_RS07330 1556349..1556480 + 132 Protein_1413 hypothetical protein -
ISA87_RS07335 1556452..1557018 - 567 WP_088717832.1 maltose O-acetyltransferase -
ISA87_RS07340 1557176..1557394 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA87_RS07345 1557421..1557795 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA87_RS07350 1558278..1561424 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA87_RS07355 1561447..1562640 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179269 WP_002892050.1 NZ_CP063898:c1557394-1557176 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179269 NZ_CP084655:c2091834-2091655 [Pectobacterium brasiliense]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACCGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAAGCAGGAATATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179269 WP_008805436.1 NZ_CP063898:c1557795-1557421 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179269 NZ_CP084655:c2091627-2091217 [Pectobacterium brasiliense]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGACGCGATTACCGGACATATCGAACTACTGATCGAGA
TGGGTCAGGATATTCCTACCGTGTCGTCAGTAGGCCAATTGGCAAAAAGCGCAGAATACGCCGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGTGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCGCTGATTGACCGAAT
TGACCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAGGCGGCATTAGAGCGAATCTCGT
CTTCCCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References