Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 356096..356721 Replicon chromosome
Accession NZ_CP063898
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISA87_RS01625 Protein ID WP_008807903.1
Coordinates 356338..356721 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA87_RS01620 Protein ID WP_004150355.1
Coordinates 356096..356338 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS01595 352086..352685 + 600 WP_064176466.1 glucose-1-phosphatase -
ISA87_RS01600 352679..353539 + 861 WP_008807907.1 virulence factor BrkB family protein -
ISA87_RS01605 353536..353973 + 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA87_RS01610 354018..354959 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
ISA87_RS01615 354974..355891 - 918 WP_032756518.1 alpha/beta hydrolase -
ISA87_RS01620 356096..356338 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA87_RS01625 356338..356721 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA87_RS01630 356895..357824 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA87_RS01635 357821..358456 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISA87_RS01640 358453..359355 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179267 WP_008807903.1 NZ_CP063898:356338-356721 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179267 NZ_CP084655:2035941-2036423 [Pectobacterium brasiliense]
ATGGGAATAACTGCGCCAGAGCCATTATCTTCCAGCCATAATCTTGCTGAATTTTGCAGCCAAGAACCGAGCTTGAATGA
ATGGCTAAAAAAGAAAGCGCTAAAAAATCATAGTGCAGGGATTTCTCGTGTATATGTGGTTTGCGCTGAAGGCACACATC
AGGTGATTGGATATTATTGTTTATCAACAGGTAGCGTTCAGAGAAATACAGCGCCTGGTGCATATAGACGTAATGCCCCC
GAATCACTACCAGTCATTGTCCTTGGGCGGCTTGCCATTGATCAGGCGTATTCTGGTAAAGGTTTGGGTGTGGCTTTATT
AAAAGACGCGATATTTCGGACTGAGAATATTGCTTTGCAAGTGGGTGTGAGGGCATTAGTCGTTCACGCGCTGGATGAGA
ACGTGCGCAATTTCTACCTTAAATTCGCTTTTGATCCCTCGCCAGTGCAGCCCCTAATGCTACTCTATCCAATTAAAATT
TAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179267 WP_004150355.1 NZ_CP063898:356096-356338 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179267 NZ_CP084655:2035672-2035950 [Pectobacterium brasiliense]
ATGAAACCTGAAACTAAAGAAGCGCCAATCAATATTCGGGCCAAAGCCTCCCAACGGGATTTGATCGATATGGCTGCAAA
CCTTGTGTCAAAATCGCGTACCGATTTTATGCTTGAAGCAGCCTGCCGCGAGGCGCAGGACATTTTGTTGGATCAACGTT
TGTTTATTTTGGATAACGAACAGTTTGACGAATTTTTGGCCGAGTTGGATGCGCCTATCACCGAAGAGCGTCAGGCTCGC
ATTAACGATCTAATGAGCCGCAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References