Detailed information of TA system TA179253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 167494..168164
• Replicon: plasmid pBS433-2
• Accession: NZ_CP063891
• Organism: Klebsiella pneumoniae strain BS433-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ISB10_RS27180
• Protein ID: WP_004213072.1
• Coordinates: 167494..167937 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ISB10_RS27185
• Protein ID: WP_004213073.1
• Coordinates: 167934..168164 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB10_RS27145	162900..163175	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ISB10_RS27150	163238..163729	+	492	WP_011251327.1	DM13 domain-containing protein	-
ISB10_RS27155	163778..164698	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ISB10_RS27160	164789..165195	+	407	Protein_178	GAF domain-containing protein	-
ISB10_RS27165	165710..166350	-	641	Protein_179	response regulator	-
ISB10_RS27170	166767..167060	+	294	Protein_180	transposase	-
ISB10_RS27175	167064..167480	+	417	Protein_181	restriction endonuclease	-
ISB10_RS27180	167494..167937	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISB10_RS27185	167934..168164	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISB10_RS27190	168772..169905	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ISB10_RS27195	169921..170214	+	294	WP_004213076.1	hypothetical protein	-
ISB10_RS27200	170204..170410	-	207	WP_004213077.1	hypothetical protein	-
ISB10_RS27205	170762..171052	+	291	WP_004213078.1	hypothetical protein	-
ISB10_RS27210	171042..171941	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..227749	227749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T179253 WP_004213072.1 NZ_CP063891:c167937-167494 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T179253 NZ_CP084654:c4577861-4577607 [Pectobacterium versatile]
ATGAATAAACGACACCAAAGAACGCTATCAGATCTGTTTTCTCGTCCAGTTAATAGTTCAATAAAATGGAGCGATATAGA
ATCTCTTTTTATTGCTCTAGGGGCTGAAATTCATGAAAGGGAAGGTTCGAGGATTGCGGTATTGTTGAAAGGTGAGAAAA
AAATTTTTCATCGACCACATCCCAGACCGACTACTGACAAAGGAGCGGTTAACTCTATTCGGGTATGGTTAGAAAGTTTA
GGAGTAAGACCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT179253 WP_004213073.1 NZ_CP063891:c168164-167934 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179253 NZ_CP084654:c4577610-4577269 [Pectobacterium versatile]
ATGATTAACACACTTCTTATCGACGGTCACACGGCCGTTATTGCTTTTGACTCGGAAATTGAAATGTTCCGGGGAGAATT
CATTGGCTTGAATGGCGGCGCTGATTTTTATGCTTACAGTGTTGATGAGCTGAAAAAAGAAGGGGCAACCTCTCTCGCTA
TTTTTCTTGATGAATGCCGCCGTGATGGTATTGAACCCTATAAATCCTACAGTGGAAAAGTCACAACACGTTTGACGCCA
GAACGTCATGCTGCGCTGGCGGTAACGGCACAAGCAACTGGAGTTTCCATTAACGAATTGTTAAACGAAGGTGTCGATCT
GGTTCTTGAACGACGCTCCTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620