Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3892028..3892647 Replicon chromosome
Accession NZ_CP063890
Organism Klebsiella pneumoniae strain BS433-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISB10_RS18735 Protein ID WP_002892050.1
Coordinates 3892028..3892246 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISB10_RS18740 Protein ID WP_002892066.1
Coordinates 3892273..3892647 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB10_RS18700 3888075..3888338 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
ISB10_RS18705 3888338..3888478 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISB10_RS18710 3888475..3889173 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
ISB10_RS18715 3889274..3890725 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
ISB10_RS18720 3890700..3891170 - 471 WP_002892026.1 YlaC family protein -
ISB10_RS18725 3891191..3891331 + 141 WP_004147370.1 hypothetical protein -
ISB10_RS18730 3891303..3891869 - 567 WP_002892030.1 maltose O-acetyltransferase -
ISB10_RS18735 3892028..3892246 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISB10_RS18740 3892273..3892647 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISB10_RS18745 3893133..3896279 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISB10_RS18750 3896302..3897495 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179249 WP_002892050.1 NZ_CP063890:c3892246-3892028 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179249 NZ_CP084654:4465599-4465850 [Pectobacterium versatile]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCTAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTTATGTCTCTGGAAGAGTATAACTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCCAATGCAAAAAGACTGATGAATTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179249 WP_002892066.1 NZ_CP063890:c3892647-3892273 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179249 NZ_CP084654:4465847-4466101 [Pectobacterium versatile]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTATCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGGTGACGCCATGATGATAGCCGCCTGC
CGCTACCACTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References