Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3300603..3301413 Replicon chromosome
Accession NZ_CP063890
Organism Klebsiella pneumoniae strain BS433-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag ISB10_RS15890 Protein ID WP_014908042.1
Coordinates 3300880..3301413 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISB10_RS15885 Protein ID WP_002887278.1
Coordinates 3300603..3300869 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB10_RS15865 3296475..3296819 - 345 WP_012737331.1 cation efflux system protein CusF -
ISB10_RS15870 3296837..3298222 - 1386 WP_012737332.1 efflux transporter outer membrane subunit -
ISB10_RS15875 3298394..3299077 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISB10_RS15880 3299067..3300500 + 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
ISB10_RS15885 3300603..3300869 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISB10_RS15890 3300880..3301413 + 534 WP_014908042.1 GNAT family N-acetyltransferase Toxin
ISB10_RS15895 3301461..3302582 - 1122 WP_012737335.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T179248 WP_014908042.1 NZ_CP063890:3300880-3301413 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179248 NZ_CP084654:4384161-4385102 [Pectobacterium versatile]
ATGGATCAGCGTGATATTTATGCCAGACAGGTCAAGCTGTTAATGACCGCCCTGCCGCATGTAGCGAAAGAGCCCTGTTT
CGCGCTGAAAGGCGGCACAGCAATTAATTTATTTGTGCAGGATTTTCCTCGTCTATCGGTAGATATCGATCTGGTTTATC
AAACCTTCATAGATCGTGATACCGATCTAGTCGCTATTGACGATGCCTTGATGCGTATTACGGAATCGCTGAACGGCAGG
CCGGGAATCACGGCGATCCTGCAGGAGAATAAAGCCGATGAAAAACGTATCATCGTTAACACCGTTGATGCGCATATAAA
GATTGAAGTCAGCCCGGTTTGGCGTGGATTGCTGTTACCTCCAGCAGAATTGCCAGTGTGCGAGCAGGTGGAAATGGAAT
TCGGCTTTACCACTATGAATGTCGTTTCACTGGCAGATCTTTACGGCGGTAAAATCTGCGCTGCGCTCGATCGCCAGCAT
CCACGCGATCTGTTTGACGTGCTAAATATGCTGGAGAAACCGGGCCTCACACGTGAGCTCTTTGATGGTTTTCTTTGCTA
TCTGGCTGGTCATCCCCGCCCCATCGCCGAACTACTGGCTCCAAACTGGGACATTGCACGTATCACAACACTGTACCAAC
AAGAATTTTCGGGCATGACGCAACAGGAAACGTCGCTGGAATCGCTTTTGTCAGTAACGATTTTACTGCCGCAAGCATTG
AGATCTCACTTTACCGTTCAGGATAAACAGTTTTTACTTAGCTATAAGCAAAACACCCCTGACTGGTCGCTATATCGTTA
TCCTGATATTCAACATCTTCCCGCTATTCGCTGGAAACAGCATAACTTATCGACCTTACATACTAAAAATCCGGCCAAAT
TTAGTGCGGCGGTAGGCAAGCTTGAGAAGGTTCTGGAACAACGTTTTAGCCCAGTCTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179248 WP_002887278.1 NZ_CP063890:3300603-3300869 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179248 NZ_CP084654:4383392-4384174 [Pectobacterium versatile]
TTGACAAATTATCTAAACTGGCTGATGCAAAACACCCTGCCCGGTCAGGTATTGCTCCAATCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTTTCTTACGTCTATGTCCAAAACGGTTGGCTCAGGCGGATCGCTCACGGCGTTTACTGCCGGACGG
GTCGTGAACCGGACTGGGTGGATGCGGTTTACTGCCTGCAAACCCAATGGGAAAAACCTGTGCGGGTGGCAGGGTTGACC
AGCCTTGCGCTACAGGGCTATTCCCATTATACCGAACTTGGTAAACCTCAAGTTTGGCTCACGCTCCCGGCTTACATAAA
GCTGCCGGGATGGTTCGCTGCCTTTGAGCCATCCGCCACCTTTGTCGTGCTCTATACATCAGGCCTGTCGGTTGATGTGA
TGCCATTCACTACAGAACTAAAAATCGGCGATCGGCGGCTGACTGGCAGCGTTCCAGAGCTTGCCGCGTATGAAATCGCC
AGTGGCGTTCCCACACAAATATCATTTGAGCATGCCGATGCATTATTTCAGGGTCTACACCTTCTTAGCCCGCGTAAATT
ACAGGCGCTACTTTCCGCCAGTCGTTCGATAAAAACCAACCGGGTGATGCTCTACCTTGCCCAGCGCAACGGACATCCTT
ATTTTCAGTATCTCGATAAAAGTGGTATAGAAATAGGCGCTGGAAAGCGGCAAATTGTCCCCGGCGGCTGGCTGGAACCG
GGTTACCAAATCACCGTTCCCAGGGAATTTAAATCGAAAGGAATCGAAGATGGATCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References