Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2663851..2664476 Replicon chromosome
Accession NZ_CP063890
Organism Klebsiella pneumoniae strain BS433-2

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag ISB10_RS12930 Protein ID WP_012737091.1
Coordinates 2664093..2664476 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISB10_RS12925 Protein ID WP_004150355.1
Coordinates 2663851..2664093 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB10_RS12900 2659842..2660441 + 600 WP_004151865.1 glucose-1-phosphatase -
ISB10_RS12905 2660435..2661295 + 861 WP_004146232.1 virulence factor BrkB family protein -
ISB10_RS12910 2661292..2661729 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISB10_RS12915 2661774..2662715 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISB10_RS12920 2662729..2663646 - 918 WP_012737090.1 alpha/beta hydrolase -
ISB10_RS12925 2663851..2664093 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISB10_RS12930 2664093..2664476 + 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
ISB10_RS12935 2664650..2665579 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISB10_RS12940 2665576..2666211 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISB10_RS12945 2666208..2667110 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T179246 WP_012737091.1 NZ_CP063890:2664093-2664476 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179246 NZ_CP084654:4134326-4134610 [Pectobacterium versatile]
ATGAGTTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGGGTGCGCCCGTACGGGAGCAGTTCAC
CAAAAAACTGAAAGAGGTTTTACAACATCCCCACGTTCCCGCCAACCGACTTCATGGAATGGCTGACTGCTACAAAATCA
AACTTCGTTCCGCTGGCTATCGGCTAGTGTATCAAGTGCTTGAACATGAGATCGTGGTGCTGGTTTTAGCTGTGGGCAAA
CGGGAACGCTCAGAAGTTTATAAAACAGCGAAAGACCGACTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179246 WP_004150355.1 NZ_CP063890:2663851-2664093 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179246 NZ_CP084654:4134085-4134336 [Pectobacterium versatile]
ATGTCCGTACAACCTATCCTTGCGAATGCTGCCGTAAGTATCAGCGACTTTAAAAAATCGCCCAATGCCGCGCTTAAGGA
AGCTAACGGCGAGCCTGTAGCTGTGCTCACCAATGGCCGTATCTCTGGCTATTATGTTTCTCCGGAAACATGGGAAGCGT
TGGCCGATTATCAGGAAGATATCGAACTTGCCAAAATCGCCCGTTCCCGGATGAAGGGGAAGCGTGTGAAGGTCGATCTG
GATGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References