Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2258480..2259066 Replicon chromosome
Accession NZ_CP063890
Organism Klebsiella pneumoniae strain BS433-2

Toxin (Protein)


Gene name doc Uniprot ID A0A330WAT7
Locus tag ISB10_RS11050 Protein ID WP_014906830.1
Coordinates 2258480..2258848 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISB10_RS11055 Protein ID WP_004174006.1
Coordinates 2258845..2259066 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB10_RS11030 2253983..2255053 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
ISB10_RS11035 2255055..2255900 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISB10_RS11040 2255897..2256784 - 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISB10_RS11045 2256891..2258207 - 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISB10_RS11050 2258480..2258848 - 369 WP_014906830.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISB10_RS11055 2258845..2259066 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISB10_RS11060 2259230..2259943 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISB10_RS11065 2259945..2260712 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISB10_RS11070 2260709..2261986 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
ISB10_RS11075 2261983..2262909 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2253246..2298355 45109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(28-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13537.95 Da        Isoelectric Point: 8.6410

>T179245 WP_014906830.1 NZ_CP063890:c2258848-2258480 [Klebsiella pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179245 NZ_CP084654:4133662-4133913 [Pectobacterium versatile]
ATGAGCTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGAGAGCTCCGATGTGGGAGCAGTTCAC
CAAAAAATTGAAAGATATTTTACAAAATCCCCACGTTCCCGCCAATCGTCTTCACGGAATGGCTGACTGCTACAAGAAGT
TTATAAAGCAGCCAAAGACCGACTATAAGCGCGGACAGTTTTCAACTCGCGGCTATTTTGTTGTGCAACCTATCTATCAA
GGTGACGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179245 WP_004174006.1 NZ_CP063890:c2259066-2258845 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179245 NZ_CP084654:4133421-4133672 [Pectobacterium versatile]
ATGTCCGTACAACCTATCCTTGCAAATGCAGCTGTGAGTATCAGCGACTTTAAAAAATCGCCCAATGCCGCGCTTAAAGA
AGCTAACGGTGAGCCTGTAGCGATGCTCACCAATGGCCGTATCTCTGGCTACTATGTTTCCCCGGAAACATGGGAGGCGC
TGGCCGACTATCAGGAAGATATCGAACTTGCCAAAATCGCCCGCTCACGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330WAT7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References