Detailed information of TA system TA179234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5274790..5276378
• Replicon: chromosome
• Accession: NZ_CP063889
• Organism: Klebsiella variicola strain BS325-3-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISB13_RS25425
• Protein ID: WP_194387132.1
• Coordinates: 5274790..5276112 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISB13_RS25430
• Protein ID: WP_008804920.1
• Coordinates: 5276112..5276378 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB13_RS25410	5269855..5272356	+	2502	WP_110196451.1	fimbrial biogenesis outer membrane usher protein	-
ISB13_RS25415	5272344..5273348	+	1005	WP_039103406.1	type 1 fimbrial protein	-
ISB13_RS25420	5273481..5274773	+	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISB13_RS25425	5274790..5276112	-	1323	WP_194387132.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISB13_RS25430	5276112..5276378	-	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISB13_RS25435	5276644..5276970	-	327	WP_194387133.1	hydroxyisourate hydrolase	-
ISB13_RS25440	5276967..5277467	-	501	WP_023297604.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISB13_RS25445	5277445..5278824	-	1380	WP_043875298.1	purine permease	-
ISB13_RS25450	5279170..5280324	+	1155	WP_194387134.1	FAD-dependent urate hydroxylase HpxO	-
ISB13_RS25455	5280305..5281228	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48969.34 Da
• Isoelectric Point: 7.9109

>T179234 WP_194387132.1 NZ_CP063889:c5276112-5274790 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADTVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESVRRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179234 NZ_CP084654:c1371816-1371472 [Pectobacterium versatile]
ATGAAATCACTCTACTGGGTTGGCAGTAGCAAAAAGGATCTCCAGTCGCTACCTGCAGATGTGCAGGATGTGTTCGGTTA
TGCACTTCATCTGGCTCAAACTGGTGGTAAACACACTCAAGCTAAGCCGCTGAAAGGTTTTGGTGGTGCGGGCGTATTAG
AAGTCGTTGAGGATTATATCGGCGATACTTATCGCGCAGTCTACACCGTCAGATTTGGAGAAGCAGTATACGTTCTACAT
GCTTTTCAGAAAAAATCGTCATCTGGAATTGCAACACAGAAACCCGATATGAACACCATTCGTGAACGCCTTAAAACCGC
AGAGAATCATGCAAAAGGAGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179234 WP_008804920.1 NZ_CP063889:c5276378-5276112 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179234 NZ_CP084654:c1371470-1371153 [Pectobacterium versatile]
ATGAGCCAGGATATTGAAGTCAGCAGCGGTAATGTATACGCCGACCTTGGTAACTTGCATGCTGAAGATATGTTGGTCAA
AGCGCAGCTTGCTACCCTGATTAGCTCAATCATCAAAAGCCGCCGTCTCACACAGGCAGAAGCAGCGAAGCTATTGGGCA
TGCCCCAACCAAAGCTATCCAACCTATTGCGTGGCCAATTCAGGGGAATCAGCGAAACCAAAATGTTGGAATGCCTTACG
AAGCTGGGACGCGATATACAGATCGTTGTAGGGAAGCCACGCCGCACACCGGGTAGCGTTAAAGTCGTGTTTGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MCN9