Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3381598..3382255 Replicon chromosome
Accession NZ_CP063889
Organism Klebsiella variicola strain BS325-3-1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISB13_RS16290 Protein ID WP_002916310.1
Coordinates 3381845..3382255 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISB13_RS16285 Protein ID WP_002916312.1
Coordinates 3381598..3381864 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB13_RS16260 3377936..3378664 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
ISB13_RS16265 3378715..3379026 + 312 WP_012540591.1 N(4)-acetylcytidine aminohydrolase -
ISB13_RS16270 3379190..3379849 + 660 WP_043876072.1 hemolysin III family protein -
ISB13_RS16275 3379984..3380322 + 339 WP_194386985.1 hypothetical protein -
ISB13_RS16280 3380369..3381352 - 984 WP_043876073.1 tRNA-modifying protein YgfZ -
ISB13_RS16285 3381598..3381864 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISB13_RS16290 3381845..3382255 + 411 WP_002916310.1 protein YgfX Toxin
ISB13_RS16295 3382262..3382783 - 522 WP_008806427.1 flavodoxin FldB -
ISB13_RS16300 3382884..3383780 + 897 WP_194386986.1 site-specific tyrosine recombinase XerD -
ISB13_RS16305 3383803..3384516 + 714 WP_194386987.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISB13_RS16310 3384522..3386255 + 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179224 WP_002916310.1 NZ_CP063889:3381845-3382255 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179224 NZ_CP084651:c86170-85847 [Yersinia ruckeri]
ATGGAGTATCTAGAATTCATTGAGACCCCCGTATTTAGCAGGCGAAGAGCTGAACTGCTGCCAGATGATGACTTCAGAGC
TTTACAGGAGCATCTTCTTAAAAACCATGATCTTGGCGACACAATCAGTAAAACAGGTGGATGTAAGAAAATCCGCTGGA
GTCGCGAAGGCATGGGGAAGCGGGGGGGAGTAAGAGTTATTTATTACGTCATAACCAAATCGGGACGCCTTTATCTGCTA
CTGGTCTATCCCAAAAACGAAAAAGACGATCTGACTGAAGCCGAAAAAGCTGTCCTGAAATCGATAAGTCAGCAAATCGA
ATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179224 WP_002916312.1 NZ_CP063889:3381598-3381864 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179224 NZ_CP084651:c85801-85505 [Yersinia ruckeri]
ATGAAAACTGAACTGTTTAATGATCTCGTTGAAAGTATGCAACAGATGGTTGCTATTGAGAAAGGTACGCTCTCTGTTCC
AGCGGAAAACATCCATCGTCATCGACTTCCAGATGTAAAATCAATGCGTGAAGCGAGCGGTCTTAAGCAAAATGAATTTG
CGGATGTAGTGGGGGTTTCTGCTTCTCTGCTCCAGAGCTGGGAACAGCATCGTCGTATTCCATCAGGTTCATCTCTAAAG
TTGTTGATGATGATAGAGCGTGACCCAGCTGTTATAGACGCTTTACGAACGCTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References