Detailed information of TA system TA179220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2159955..2161636
• Replicon: chromosome
• Accession: NZ_CP063889
• Organism: Klebsiella variicola strain BS325-3-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: ISB13_RS10375
• Protein ID: WP_032734038.1
• Coordinates: 2159955..2160872 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISB13_RS10380
• Protein ID: WP_032734037.1
• Coordinates: 2160878..2161636 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB13_RS10355	2155261..2156781	+	1521	WP_064324014.1	sugar ABC transporter ATP-binding protein	-
ISB13_RS10360	2156778..2157776	+	999	WP_004206363.1	ribose ABC transporter permease	-
ISB13_RS10365	2157807..2158748	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISB13_RS10370	2158823..2159830	+	1008	WP_022065799.1	carbohydrate kinase family protein	-
ISB13_RS10375	2159955..2160872	-	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISB13_RS10380	2160878..2161636	-	759	WP_032734037.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISB13_RS10385	2161721..2162449	-	729	WP_194387312.1	response regulator transcription factor	-
ISB13_RS10390	2162722..2164869	+	2148	WP_194387313.1	formate dehydrogenase subunit alpha	-
ISB13_RS10395	2165223..2165909	+	687	WP_016160120.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T179220 WP_032734038.1 NZ_CP063889:c2160872-2159955 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179220 NZ_CP084650:1928025-1928483 [Yersinia ruckeri]
GTGATGCTCTACCGGATAGTTCACAACCGTTACCTGGCAACGGCCTGGACCGGATATGGCGCAGAAACCTATGGTGGCCG
CTGGAATCATAAAGGCCATGCGGCGATTTATCTGGCCAGTAGCGTTTCTCTGGCGATGCTCGAAACACTGGTGCATGTAG
AAGATAGCGCGACGCTGAACGATTTTAATCTATTTCAGATAACCATTCCCGATAATGCAATTATGGTACTGCAACAGCAA
GATTGGCCACGTGGATGGCGAGATGACCCCACCCCCGTGGCCACCATGGACATCGGCACCGAATGGATTGGTTCAGTATC
ATCTGTCGGTCTGCTGGTACCATCCACGTTAGTCCCTTTGGAAAGAAATATGCTGGTGAATCCTAAACACCGCGATTTCG
CCTCGTGCCTGAAAAGCATCAATCCACTGACGTTCAGTTTTGATCCTCGTCTAAAGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28693.08 Da
• Isoelectric Point: 10.2152

>AT179220 WP_032734037.1 NZ_CP063889:c2161636-2160878 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179220 NZ_CP084650:1927565-1928011 [Yersinia ruckeri]
ATGAGAACTTATCACCCAAGCCCGATGGCCAAAGTCAGCGCACTATGGCGTGAGATTGGTCTACCCGCCAGTCGTGGCAC
CGCTTTGGTGGACAGCATAAAACTGGGATTTACTGTGGATGTGATCGACACTATACATTTATGGGCTGATATGCCTAAAT
CTGAAATATTACGCGCTACGGGCATTCCGAGCCGCAGTCTGACGCGCCGTCGTACCCATGATGGGCGTTTTACCCCGGAA
GAAAGCGAACGTATTGCACGGTTTGTCCGGGTTATGGATGCGGCGGTAGATTTGTTCGGTGGTGACCGCACCAAAGCAAC
CGCATGGATGTCGATGCCGGTCAAAGGGCTGGGTAATCGAACGCCCGATTCACTGCTGGAAACCGAAACCGGTGCGTTGG
AAATCTGCGACCTGATTGGTCGTCTCGAACATGGGGTCTTTTCGTGA

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2