Detailed information of TA system TA179217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 93585..94321
• Replicon: plasmid pBM419-3
• Accession: NZ_CP063888
• Organism: Klebsiella quasipneumoniae strain BM419-3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ISB14_RS26315
• Protein ID: WP_003026803.1
• Coordinates: 93839..94321 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISB14_RS26310
• Protein ID: WP_003026799.1
• Coordinates: 93585..93851 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB14_RS26265	89647..90009	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
ISB14_RS26270	90059..90409	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ISB14_RS26275	90767..91036	+	270	WP_019706036.1	hypothetical protein	-
ISB14_RS26280	91024..91599	+	576	WP_004152103.1	hypothetical protein	-
ISB14_RS26285	91630..92124	+	495	WP_004152104.1	DNA-binding protein	-
ISB14_RS26290	92168..92536	+	369	WP_004152105.1	hypothetical protein	-
ISB14_RS26295	92570..92773	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
ISB14_RS26300	92822..93079	+	258	WP_004152107.1	hypothetical protein	-
ISB14_RS26305	93155..93409	+	255	WP_004152108.1	hypothetical protein	-
ISB14_RS26310	93585..93851	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISB14_RS26315	93839..94321	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ISB14_RS26320	94522..95925	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
ISB14_RS26325	95954..96586	-	633	WP_001567369.1	hypothetical protein	-
ISB14_RS26330	96763..98017	-	1255	Protein_95	IS3 family transposase	-
ISB14_RS26335	98105..99067	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..163716	163716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T179217 WP_003026803.1 NZ_CP063888:93839-94321 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179217 NZ_CP084649:1-324 [Yersinia ruckeri]
ATGGAGTATCTAGAATTCATTGAGACCCCCGTATTTAGCAGGCGAAGAGCTGAACTGCTGCCAGATGATGACTTCAGAGC
TTTACAGGAGCATCTTCTTAAAAACCATGATCTTGGCGACACAATCAGTAAAACAGGTGGATGTAAGAAAATCCGCTGGA
GTCGCGAAGGCATGGGGAAGCGGGGGGGAGTAAGAGTTATTTATTACGTCATAACCAAATCGGGACGCCTTTATCTGCTA
CTGGTCTATCCCAAAAACGAAAAAGACGATCTGACTGAAGCCGAAAAAGCTGTCCTGAAATCGATAAGTCAGCAAATCGA
ATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179217 WP_003026799.1 NZ_CP063888:93585-93851 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179217 NZ_CP084649:370-666 [Yersinia ruckeri]
ATGAAAACTGAACTGTTTAATGATCTCGTTGAAAGTATGCAACAGATGGTTGCTATTGAGAAAGGTACGCTCTCTGTTCC
AGCGGAAAACATCCATCGTCATCGACTTCCAGATGTAAAATCAATGCGTGAAGCGAGCGGTCTTAAGCAAAATGAATTTG
CGGATGTAGTGGGGGTTTCTGCTTCTCTGCTCCAGAGCTGGGAACAGCATCGTCGTATTCCATCAGGTTCATCTCTAAAG
TTGTTGATGATGATAGAGCGTGACCCAGCTGTTATAGACGCTTTACGAACGCTTTAG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66