Detailed information of TA system TA179204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 71404..72047
• Replicon: plasmid pBS361-1
• Accession: NZ_CP063886
• Organism: Klebsiella pneumoniae strain BS361-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ISB15_RS26195
• Protein ID: WP_186474563.1
• Coordinates: 71631..72047 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: ISB15_RS26190
• Protein ID: WP_001261276.1
• Coordinates: 71404..71634 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB15_RS26150	66816..66929	+	114	WP_014343462.1	hypothetical protein	-
ISB15_RS26155	67058..67315	+	258	WP_194429505.1	hypothetical protein	-
ISB15_RS26160	67373..68152	-	780	WP_194429504.1	site-specific integrase	-
ISB15_RS26165	68350..69366	-	1017	WP_194429503.1	hypothetical protein	-
ISB15_RS26170	69363..69686	-	324	WP_004197641.1	hypothetical protein	-
ISB15_RS26175	69713..70114	-	402	WP_072199347.1	hypothetical protein	-
ISB15_RS26180	70282..70587	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
ISB15_RS26185	70589..70807	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
ISB15_RS26190	71404..71634	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISB15_RS26195	71631..72047	+	417	WP_186474563.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISB15_RS26200	72088..72966	-	879	WP_064182004.1	restriction endonuclease	-
ISB15_RS26205	73627..74904	+	1278	Protein_91	HlyD family secretion protein	-
ISB15_RS26210	74894..77002	+	2109	WP_114473038.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	-	1..104834	104834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T179204 WP_186474563.1 NZ_CP063886:71631-72047 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T179204 NZ_CP084647:458958-459908 [Yersinia ruckeri]
ATGGATGATTTAACAAAGCTATTCCCAGATGTTGCTGATGCTCTGGGCATTGAATCTGTTTCAATTGTAGAAAAAGATTA
TTATATCGTTGAGTTATTACGATTGCTCCAGCCTATGGACTTTGACACGCATCAGCTGGTTTTTGCCGGTGGAACAGCAT
TGTCAAAAGCGGGTATATCTTTGAACAGGATGTCGGAAGACGTGGACATTAAGCTTGTCCCCGTCGCTGGTTTTACCCAG
CAATATTCCAGAGGCCAGCGTAAAGACATTCGTAAGGGTATTATTCGGACCGTTATCGAAACGATTACAGCTTCAGGTAC
GTTTCGCATGGATGAAGGGTATCCAAAGGTTACTCGTGATGAATATCGCTATAATGAAATTCCGGTGCGTTACCCGCAGG
AATATGTACAAGTTCCCTGCTTAAGGCCATTTATCAAACTGGAACTGATGGAAACGGATTTGCTTGAGGCTGCGGAAAAT
CGTGCTATTAGCTCCTTGGTAATGGATCTAACAAAGAAGGGCGAAGTAGTGAGGGCTTTCCCTTGCGCAACCATCGGTAG
CACGCAGGCTGAAAAACTGATCTCGATGATGCGCAGAACGGCTGCAAGTATGCGTGATGTGGAACGTGCAGTGGATGAAT
CACTGGTACGTCATATCTACGATAATTTTTGTATTGTTAGAGAAAAAGGTGCTGACGTTCCTGTTTTGAAGCAATTCGTG
CAGGAATGCATTGAACAGGATATTGAACGTTACGGTGCTCAGTACCCTGAATTCTGCGAGTCGCCTGTCGAAGAACTAAA
AATGGGGCTGGAAGAACTCGTGAGCAATCCTGTTTATAAGGAACGCTATCTGCAATTCGTCACACCAATGGTCTTCGGTG
AAAGCCACGTTTCATGGGAAGAGGCCTATGCCTGTTTTCGCAAAACGGTATTGGATGTGGTTGATGCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT179204 WP_001261276.1 NZ_CP063886:71404-71634 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179204 NZ_CP084647:458570-458965 [Yersinia ruckeri]
ATGACTACAAAAGCTCGTATTCAGTTACGACTGAAACGCTCAAAGCGTTATGTTTTCACTCGTGACGATTTCAAAGATCT
TGCTGGCTACGACCAAATTGGTAGAGCACTGCGTGAACTGGTGAAGGAAGGGCAGCTGCTGAAAGTAGGCTATGGCGTCT
ACACCAAAGCGCGGCAAAATGTGATTACCGGTAAGTTGATGCCTGCGGCTCCCGGAGGTTCGGCTGCGGTAGTGATTGAG
GCGTTGGAACGTCTGAAAGTGGATTACCGCTTGGTTGGAGCAACAAGCGCCTATAATAGCGGTAAATCCACTCAGATCCC
CGCTTCACTGGAAATCAAAACCTCACCACGTTTTAAGAGGACATTGTCAGTAGGGAGCAGCAAACTAAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023