Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70282..70807 Replicon plasmid pBS361-1
Accession NZ_CP063886
Organism Klebsiella pneumoniae strain BS361-1

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag ISB15_RS26180 Protein ID WP_004197633.1
Coordinates 70282..70587 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag ISB15_RS26185 Protein ID WP_006788213.1
Coordinates 70589..70807 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB15_RS26145 65414..66241 - 828 WP_194429506.1 replication initiation protein -
ISB15_RS26150 66816..66929 + 114 WP_014343462.1 hypothetical protein -
ISB15_RS26155 67058..67315 + 258 WP_194429505.1 hypothetical protein -
ISB15_RS26160 67373..68152 - 780 WP_194429504.1 site-specific integrase -
ISB15_RS26165 68350..69366 - 1017 WP_194429503.1 hypothetical protein -
ISB15_RS26170 69363..69686 - 324 WP_004197641.1 hypothetical protein -
ISB15_RS26175 69713..70114 - 402 WP_072199347.1 hypothetical protein -
ISB15_RS26180 70282..70587 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
ISB15_RS26185 70589..70807 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISB15_RS26190 71404..71634 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISB15_RS26195 71631..72047 + 417 WP_186474563.1 type II toxin-antitoxin system VapC family toxin -
ISB15_RS26200 72088..72966 - 879 WP_064182004.1 restriction endonuclease -
ISB15_RS26205 73627..74904 + 1278 Protein_91 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..104834 104834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T179203 WP_004197633.1 NZ_CP063886:c70587-70282 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179203 NZ_CP084647:455120-455776 [Yersinia ruckeri]
GTGGCCTATGACTACAGCACTAGCCTCAACCCGGAAAAAGCCTTGATCTGGCGCATTGTCCATCGGGACAATATTCCGTG
GATACTGGACAACGGTCTGCACTGTGGCAACAGTCCGGTGCAGGCGGAAGACTGGATTAATATCGGTAACCCGGAGCTTA
TCGACAAACGTGCCGGACATCTGGTGCCAACCGGAATGCGGGGGACGCTGCATGACTATGTGCCGTTTTACTTTACTCCT
TTCTCGCCGATGCTGATGAATATCCGCAGCGGACGTGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGTAGCCTGCGAAACATGGTGGCGCAAAATGTCTCCTTCGTGTTTACCGATAGCCACGCATATTATAATCTGGCTAATT
ACTTTACCGAGCTGGACAGTCTGAACCAGATCGATTGGCCCATTCTACAAGCGCGGGATTTCCGTCGCGATCAGGACGAT
TTGGCCAAGTTCGAACGCTATCAGGCAGAGGCGTTAATTTGGAAGCACTGCCCAATAGCGCTGCTTGGCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGTTGTCGCAACGCAATCTGTCAATGCCCGTTCACGCGCGGGCTG
GGTGGTACTTCTCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT179203 WP_006788213.1 NZ_CP063886:c70807-70589 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179203 NZ_CP084647:455773-456840 [Yersinia ruckeri]
ATGATTACATTTACACAAGGCAATTTACTGGATGCGCCCGTAGAAGCACTAGTGAATACGGTTAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTCAAAGAGCGCTTCCCCGTCAATATGAAAGCCTATGCTCTCGCCTGCAAACATAAGC
AGGTAATGACAGGTAAAATGTTTATTACCGAAACCGGCGAACTGATGGGGCCTCGCTGGATTGTCAACTTCCCAACTAAA
CAACACTGGCGCGCAGACTCCCGCATGGAGTGGGTTGAAGACGGCTTGCAGGATTTACGCCGCTTTTTGATCAAAGAGAA
AGTGCAATCTATCGCCATTCCCCCACTGGGGGCGGGTAACGGCGGCCTGAATTGGTCGGACGTTCGGATGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATTCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGTACC
GGCGTAAAAAAGCTCACTCCCGCCAGAGCGCTGATTGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGTTACAACGCGCCATTGAACAGCATCAGCAGGAAGATGTGCTGAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTACGCCCCCAACCTTAACCATCTGCTTAACGCGCTAGATGGCACGTATCTGAAA
GCAGAGAAACGCATCCCGGACACCCAGCCGCTGGATGTGATCTGGTTTAATGATCAGGAAAAAGAACACATCAACCTCTG
GCTGAACAACGAGGCCAAAGAGTGGCTACCGGCGCTTGAGCAGGTCAGTCAGTTGATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGCTGCTGGCAACCGTTGACTGGCTGCTGACCCGAGGAAAATGCCAGCCCACGCTGGATTCCGTGAAAGAGGGG
CTGCACCAGTGGCCTGCGGGTGAACGCTGGGCCAGCCGTAAGATGAAGCTCTTTGACGACAAGAATCTGCAATTTGCCAT
CAACCGCGTGATGGAGTTCCATTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References