Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2547478..2548288 Replicon chromosome
Accession NZ_CP063885
Organism Klebsiella pneumoniae strain BS361-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISB15_RS12265 Protein ID WP_004178461.1
Coordinates 2547755..2548288 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISB15_RS12260 Protein ID WP_002887278.1
Coordinates 2547478..2547744 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB15_RS12240 2543349..2543693 - 345 WP_002887266.1 cation efflux system protein CusF -
ISB15_RS12245 2543711..2545096 - 1386 WP_032413315.1 efflux transporter outer membrane subunit -
ISB15_RS12250 2545269..2545952 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISB15_RS12255 2545942..2547375 + 1434 WP_032413318.1 Cu(+)/Ag(+) sensor histidine kinase -
ISB15_RS12260 2547478..2547744 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISB15_RS12265 2547755..2548288 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
ISB15_RS12270 2548336..2549457 - 1122 WP_016946712.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179192 WP_004178461.1 NZ_CP063885:2547755-2548288 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179192 NZ_CP084643:469340-470290 [Yersinia ruckeri]
ATGGATGATTTAACAAAGCTATTCCCAGATGTTGCTGATGCTCTGGGCATTGAATCTGTTTCAATTGTAGAAAAAGATTA
TTACATCGTTGAGTTATTACGATTGCTCCAGCCTATGGACTTTGACACGCATCAGCTGGTTTTTGCCGGTGGAACAGCAT
TGTCAAAAGCGGGTATATCTTTGAACAGGATGTCGGAGGACGTGGACATTAAGCTTGTACCTCTCGCCGGTTTTTCCCAG
CAATATTCCAGAGGTCAGCGTAAAAGCATCCGTAAGGGTATTATTCAGACCATTATCGAGACGGTTGCCGCCTCAGGTAC
GTTTCTCCTGGATGAAGAGTATCCAAAGGTTACTCGTGATGAATACCGTTATAATGAAATTCCGGTGCGTTACCCGCAGG
AATATGTACAAGTTCCCTGCTTAAGGCCATTTATCAAACTGGAACTGATGGAAACGGATTTGCTTGAGGCTGCGGAAAAT
CGTGCTATTAGCTCCTTGGTAATGGATCTAACAAAGAAGGGCGAAGTAGTGAGGGCTTTCCCTTGCGCAACCATCGGTAG
CACGCAGGCTGAAAAACTGATCTCGATGATGCGCAGAACGGCTGCAAGTATGCGTGATGTGGAACGTGCAGTGGATGAAT
CACTGGTACGTCATATCTACGATAATTTTTGTATTGTTAGAGAAAAAGGTGCTGACGTTCCTGTTTTGAAGCAATTCGTG
CAGGAATGCATTGAACAGGATATTGAACGTTACGGTGCTCAGTACCCTGAATTCTGCGAGTCGCCTGTCGAAGAACTAAA
AATGGGGCTGGAAGAACTCGTGAGCAATCCTGTTTATAAGGAACGCTATCTGCAATTCGTCACACCAATGGTCTTCGGTG
AAAGCCACGTTTCATGGGAAGAGGCCTATGCCTGTTTTCGCAAAACGGTATTGGATGTGGTTGATGCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179192 WP_002887278.1 NZ_CP063885:2547478-2547744 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179192 NZ_CP084643:468952-469347 [Yersinia ruckeri]
ATGACTACAAAAGCTCGTATTCAGTTACGACTGAAACGCTCAAAGCGTTATGTTTTCACTCGTGACGATTTCAAAGATCT
TGCTGGCTACGACCAAATTGGTAGAGCACTGCGTGAACTGGTGAAGGAAGGGCAGCTGCTGAAAGTAGGCTATGGCGTCT
ACACCAAAGCGCGGCAAAATGTGATTACCGGTAAGTTGATGCCTGCGGCTCCCGGAGGTTCGGCTGCGGTAGTGATTGAG
GCGTTGGAACGTCTGAAAGTGGATTACCGCTTGGTTGGAGCAACAAGCGCCTATAATAGCGGTAAATCCACTCAGATCCC
CGCTTCACTGGAAATCAAAACCTCACCACGTTTTAAGAGGACATTGTCAGTAGGGAGCAGCAAACTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References