Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1959029..1959654 Replicon chromosome
Accession NZ_CP063885
Organism Klebsiella pneumoniae strain BS361-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISB15_RS09470 Protein ID WP_002882817.1
Coordinates 1959271..1959654 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISB15_RS09465 Protein ID WP_004150355.1
Coordinates 1959029..1959271 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB15_RS09440 1955019..1955618 + 600 WP_004151865.1 glucose-1-phosphatase -
ISB15_RS09445 1955612..1956472 + 861 WP_004146232.1 virulence factor BrkB family protein -
ISB15_RS09450 1956469..1956906 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISB15_RS09455 1956951..1957892 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISB15_RS09460 1957907..1958824 - 918 WP_012737090.1 alpha/beta hydrolase -
ISB15_RS09465 1959029..1959271 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISB15_RS09470 1959271..1959654 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISB15_RS09475 1959828..1960757 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISB15_RS09480 1960754..1961389 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISB15_RS09485 1961386..1962288 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179188 WP_002882817.1 NZ_CP063885:1959271-1959654 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179188 NZ_CP084642:c3180626-3179970 [Yersinia ruckeri]
GTGGCCTATGACTACAGCACTAGCCTCAACCCGGAAAAAGCCTTGATCTGGCGCATTGTCCATCGGGACAATATTCCGTG
GATACTGGACAACGGTCTGCACTGTGGCAACAGTCCGGTGCAGGCGGAAGACTGGATTAATATCGGTAACCCGGAGCTTA
TCGACAAACGTGCCGGACATCTGGTGCCAACCGGAATGCGGGGGACGCTGCATGACTATGTGCCGTTTTACTTTACTCCT
TTCTCGCCGATGCTGATGAATATCCGCAGCGGACGTGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGTAGCCTGCGAAACATGGTGGCGCAAAATGTCTCCTTCGTGTTTACCGATAGCCACGCATATTATAACCTGGCTAATT
ACTTTACCGAGCTGGACAGTCTGAACCAGATCGATTGGCCCATTCTACAAGCGCGGGATTTCCGTCGCGATCAGGACGAT
TTGGCCAAGTTCGAACGCTATCAGGCAGAGGCATTAATTTGGAAGCACTGCCCAATAGCGCTGCTTGGCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGTTGTCGCAACGCAATCTGTCAATGCCCGTTCACGCGCGGGCTG
GGTGGTACTTCTCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179188 WP_004150355.1 NZ_CP063885:1959029-1959271 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179188 NZ_CP084642:c3179973-3178906 [Yersinia ruckeri]
ATGATTACATTTACACAAGGCAATTTACTGGATGCGCCCGTAGAAGCACTAGTGAATACGGTTAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTCAAAGAGCGCTTCCCCGTCAATATGAAAGCCTATGCTCTCGCCTGCAAACATAAGC
AGGTAATGACAGGTAAAATGTTTATTACCGAAACCGGCGAACTGATGGGGCCTCGCTGGATTGTCAACTTCCCAACTAAA
CAACACTGGCGCGCAGACTCCCGCATGGAGTGGGTTGAAGACGGCTTGCAGGATTTACGCCGCTTTTTGATCAAAGAGAA
AGTGCAATCTATCGCCATTCCCCCACTGGGGGCGGGTAACGGCGGCCTGAATTGGTCGGACGTTCGGATGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATTCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCACC
GGCGTAAAAAAGCTCACTCCCGCCAGAGCGCTGATTGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGTTACAACGCGCCATTGAACAGCATCAGCAGGAAGATGTGCTGAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTACGCCCCCAACCTTAACCATCTGCTTAACGCGCTAGATGGCACGTATCTGAAA
GCAGAGAAACGCATCCCGGACACCCAGCCGCTGGATGTGATCTGGTTTAATGATCAGGAAAAAGAACACATCAACCTCTG
GCTGAACAACGAGGCCAAAGAGTGGCTACCGGCGCTTGAGCAGGTCAGTCAGTTGATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGCTGCTGGCAACCGTTGACTGGCTGCTGACCCGAGGAAAATGCCAGCCCACGCTGGATTCCGTGAAAGAGGGG
CTGCACCAGTGGCCTGCGGGTGAACGCTGGGCCAGCCGTAAGATGAAGCTCTTTGACGACAAGAATCTGCAATTTGCCAT
CAACCGCGTGATGGAGTTCCATTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References