Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 129693..130283 Replicon chromosome
Accession NZ_CP063885
Organism Klebsiella pneumoniae strain BS361-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag ISB15_RS00750 Protein ID WP_022615588.1
Coordinates 129693..130025 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag ISB15_RS00755 Protein ID WP_000288812.1
Coordinates 130026..130283 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB15_RS00725 125740..125985 + 246 WP_004141189.1 signal transduction protein PmrD -
ISB15_RS00730 126115..127569 + 1455 WP_023301083.1 AMP nucleosidase -
ISB15_RS00740 127935..129371 - 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISB15_RS00750 129693..130025 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISB15_RS00755 130026..130283 - 258 WP_000288812.1 antitoxin Antitoxin
ISB15_RS00760 130618..130824 - 207 WP_032412591.1 helix-turn-helix domain-containing protein -
ISB15_RS00765 130821..131282 - 462 WP_032412594.1 hypothetical protein -
ISB15_RS00770 131591..132592 - 1002 WP_032412597.1 DUF4917 family protein -
ISB15_RS00775 132864..134099 + 1236 WP_021466313.1 DNA (cytosine-5-)-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T179184 WP_022615588.1 NZ_CP063885:c130025-129693 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T179184 NZ_CP084642:1570302-1570395 [Yersinia ruckeri]
TAAGCCTGCATGAAATGCCAACTTTTAGCGCACGGCTCTATCCCAAGAGCCATTTCCCTGGACCGAATATAGGATTCGTA
TTCGGTCTTTTTTT

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT179184 WP_000288812.1 NZ_CP063885:c130283-130026 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179184 NZ_CP084642:c1570399-1570253 [Yersinia ruckeri]
AGATAAAAAAAGACCGAATACGAATCCTATATTCGGTCCAGGGAAATGGCTCTTGGGATAGAGCCGTGCGCTAAAAGTTG
GCATTTCATGCAGGCTTATTAAGCCGTACCACTTAAGCGTAGTAGACGACCCACATTTTACCAATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References