Detailed information of TA system TA179183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 80371..81013
• Replicon: chromosome
• Accession: NZ_CP063885
• Organism: Klebsiella pneumoniae strain BS361-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ISB15_RS00450
• Protein ID: WP_075647414.1
• Coordinates: 80840..81013 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISB15_RS00445
• Protein ID: WP_075647415.1
• Coordinates: 80371..80781 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB15_RS00405	75597..76031	-	435	WP_012542168.1	phage terminase small subunit P27 family	-
ISB15_RS00410	76281..76712	-	432	WP_081277328.1	hypothetical protein	-
ISB15_RS00415	76709..77032	-	324	WP_194429326.1	hypothetical protein	-
ISB15_RS00420	76984..77346	-	363	WP_017880264.1	HNH endonuclease	-
ISB15_RS00425	77330..77536	-	207	WP_023159883.1	hypothetical protein	-
ISB15_RS00430	78473..78818	-	346	Protein_85	hypothetical protein	-
ISB15_RS00435	78815..79312	-	498	WP_075647416.1	lysozyme	-
ISB15_RS00440	79312..79527	-	216	WP_017880269.1	class II holin family protein	-
ISB15_RS00445	80371..80781	-	411	WP_075647415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISB15_RS00450	80840..81013	-	174	WP_075647414.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISB15_RS00455	81279..82141	+	863	Protein_90	hypothetical protein	-
ISB15_RS00460	82404..82820	+	417	WP_130943902.1	hypothetical protein	-
ISB15_RS00465	82836..83201	-	366	WP_075647411.1	DUF1133 family protein	-
ISB15_RS00470	83220..84200	-	981	WP_075647410.1	DUF968 domain-containing protein	-
ISB15_RS00475	84225..85034	-	810	WP_075647409.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
ISB15_RS00480	85031..85929	-	899	Protein_95	conserved phage C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	890..90990	90100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (86-128)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6603.75 Da
• Isoelectric Point: 11.3260

>T179183 WP_075647414.1 NZ_CP063885:c81013-80840 [Klebsiella pneumoniae]
VKQREFQRWLESQGVEITDGAKHLKLRLNGKRSVMPRHPGSEIVEQLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T179183 NZ_CP084642:687392-687814 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15269.40 Da
• Isoelectric Point: 4.4249

>AT179183 WP_075647415.1 NZ_CP063885:c80781-80371 [Klebsiella pneumoniae]
MRYPVNLSKAEEGGFFVEFPDIPEALTQGDTKEEALDNALDALVTAFEFYFEDGDRIPEPGVPTGDYVELPLSIEAKVLM
LNAFIESKLSQTELAAKMNVKKQEVTRLFDLKHSTKIDTIQKAVNSLGHRMELKYA

Nucleotide

• Length: 411 bp

>AT179183 NZ_CP084642:687145-687411 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA