Detailed information of TA system TA179182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 26899..27541
• Replicon: chromosome
• Accession: NZ_CP063885
• Organism: Klebsiella pneumoniae strain BS361-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ISB15_RS00145
• Protein ID: WP_075647414.1
• Coordinates: 27368..27541 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISB15_RS00140
• Protein ID: WP_075647415.1
• Coordinates: 26899..27309 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB15_RS00100	22112..22546	-	435	WP_012542168.1	phage terminase small subunit P27 family	-
ISB15_RS00105	22796..23227	-	432	WP_081277328.1	hypothetical protein	-
ISB15_RS00110	23224..23547	-	324	WP_194429326.1	hypothetical protein	-
ISB15_RS00115	23499..23861	-	363	WP_017880264.1	HNH endonuclease	-
ISB15_RS00120	23845..24051	-	207	WP_023159883.1	hypothetical protein	-
ISB15_RS00125	24995..25345	-	351	WP_016160650.1	hypothetical protein	-
ISB15_RS00130	25342..25839	-	498	WP_194443761.1	lysozyme	-
ISB15_RS00135	25839..26053	-	215	Protein_26	class II holin family protein	-
ISB15_RS00140	26899..27309	-	411	WP_075647415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISB15_RS00145	27368..27541	-	174	WP_075647414.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISB15_RS00150	27807..28668	+	862	Protein_29	hypothetical protein	-
ISB15_RS00155	28929..29345	+	417	WP_130943902.1	hypothetical protein	-
ISB15_RS00160	29361..29726	-	366	WP_075647411.1	DUF1133 family protein	-
ISB15_RS00165	29745..30725	-	981	WP_075647410.1	DUF968 domain-containing protein	-
ISB15_RS00170	30750..31559	-	810	WP_075647409.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
ISB15_RS00175	31556..32470	-	915	WP_040188691.1	conserved phage C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	890..90990	90100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (86-128)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6603.75 Da
• Isoelectric Point: 11.3260

>T179182 WP_075647414.1 NZ_CP063885:c27541-27368 [Klebsiella pneumoniae]
VKQREFQRWLESQGVEITDGAKHLKLRLNGKRSVMPRHPGSEIVEQLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T179182 NZ_CP084641:c3276772-3276482 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAATCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTGGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATATTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15269.40 Da
• Isoelectric Point: 4.4249

>AT179182 WP_075647415.1 NZ_CP063885:c27309-26899 [Klebsiella pneumoniae]
MRYPVNLSKAEEGGFFVEFPDIPEALTQGDTKEEALDNALDALVTAFEFYFEDGDRIPEPGVPTGDYVELPLSIEAKVLM
LNAFIESKLSQTELAAKMNVKKQEVTRLFDLKHSTKIDTIQKAVNSLGHRMELKYA

Nucleotide

• Length: 411 bp

>AT179182 NZ_CP084641:c3277016-3276762 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTAATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA