Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3124982..3125823 Replicon chromosome
Accession NZ_CP063881
Organism Klebsiella pneumoniae strain S90-2

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag IOD49_RS15270 Protein ID WP_000854822.1
Coordinates 3125440..3125823 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M0CWL2
Locus tag IOD49_RS15265 Protein ID WP_053271974.1
Coordinates 3124982..3125350 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD49_RS15230 3120216..3121121 + 906 Protein_2966 IS4-like element ISVsa5 family transposase -
IOD49_RS15235 3121170..3121874 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
IOD49_RS15240 3121939..3122400 + 462 Protein_2968 autotransporter domain-containing protein -
IOD49_RS15245 3122727..3123545 + 819 WP_001234702.1 DUF945 domain-containing protein -
IOD49_RS15250 3123636..3124121 + 486 WP_000214317.1 antirestriction protein -
IOD49_RS15255 3124136..3124612 + 477 WP_001186747.1 RadC family protein -
IOD49_RS15260 3124681..3124902 + 222 WP_000692312.1 DUF987 domain-containing protein -
IOD49_RS15265 3124982..3125350 + 369 WP_053271974.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IOD49_RS15270 3125440..3125823 + 384 WP_000854822.1 type IV toxin-antitoxin system toxin CbtA Toxin
IOD49_RS15275 3125814..3126302 + 489 WP_001177592.1 hypothetical protein -
IOD49_RS15280 3126432..3126836 + 405 WP_003031976.1 IS66 family insertion sequence hypothetical protein -
IOD49_RS15285 3126833..3127180 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
IOD49_RS15290 3127229..3128762 + 1534 Protein_2978 IS66-like element ISKpn24 family transposase -
IOD49_RS15295 3128759..3128968 + 210 WP_200974028.1 hypothetical protein -
IOD49_RS15300 3129065..3129910 + 846 WP_001280435.1 DUF4942 domain-containing protein -
IOD49_RS15310 3130209..3130715 + 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage aac(3)-IId - 3106625..3134620 27995
- inside IScluster/Tn aac(3)-IId - 3114749..3128762 14013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T179168 WP_000854822.1 NZ_CP063881:3125440-3125823 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T179168 NZ_CP084638:c56864-56574 [Yersinia ruckeri]
ATGATTAAGTCTTTTCGGGATAAAAACCTAGAACTGTTCTATATGGAAAGTAAACGAGATCGCGCGATACCGGCAACAAT
AGAGCGCCAGTTAGCGAAGAAGCTTGATATGTTGGCCGCCGCACATAATGAGCGAGATTTGTTTATTCCAACCAGTAATT
ACTACAAGCGTCTCTCAGGGCAGTTTGATGGCTGGTCAAGTATGAGAGTCAATATTCAATGGCGGTTAATGTTTCAATGG
AAAGACGGATATGCTGAAAGCATCTACCTCGATCCTCATCAAGACACCTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13679.41 Da        Isoelectric Point: 6.4758

>AT179168 WP_053271974.1 NZ_CP063881:3124982-3125350 [Klebsiella pneumoniae]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT179168 NZ_CP084638:c56562-56233 [Yersinia ruckeri]
ATGCTTGATACCACTACTCGCGAACCTACTACGGTAGGCGAAATTTTGCAGGAAGAATTTTTAACCCCATTAGCCATTGG
GCAGGAAGAACTGGCCGATATTATGGGCGTGACTCGCGCAACGGTTAACCGTTTGTGCAAAGGCCGTCGTCGTCTGACTG
TAGAGGAAGCGACGCTGTTTTCTGAGTTGTTTGAGACGACGCCCGAATTTTGGTTGAACCTCCAAGCCGCTCATGACCGT
TGGGAAGCACGAGAAGCGCTTCAAAAACATAAGCGACCCCCAATGCGTCCTATCATGGAAATGTTGGGACGGCGTATCTC
ACACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CWL2

References