Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1769629..1770439 Replicon chromosome
Accession NZ_CP063881
Organism Klebsiella pneumoniae strain S90-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IOD49_RS08685 Protein ID WP_002887280.1
Coordinates 1769629..1770162 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IOD49_RS08690 Protein ID WP_002887278.1
Coordinates 1770173..1770439 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD49_RS08680 1768460..1769581 + 1122 WP_002887282.1 cupin domain-containing protein -
IOD49_RS08685 1769629..1770162 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
IOD49_RS08690 1770173..1770439 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IOD49_RS08695 1770542..1771975 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
IOD49_RS08700 1771965..1772648 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IOD49_RS08705 1772820..1774205 + 1386 WP_102001188.1 efflux transporter outer membrane subunit -
IOD49_RS08710 1774223..1774567 + 345 WP_023287080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T179161 WP_002887280.1 NZ_CP063881:c1770162-1769629 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179161 NZ_CP084635:1955160-1955618 [Yersinia ruckeri]
GTGATGCTCTACCGGATAGTTCACAACCGTTACCTGGCAACGGCCTGGACCGGATATGGCGCAGAAACCTATGGTGGCCG
CTGGAATCATAAAGGCCATGCGGCGATTTATCTGGCCAGTAGCGTTTCTCTGGCGATGCTCGAAACACTGGTGCATGTAG
AAGATAGCGCGACGCTGAACGATTTTAATCTATTTCAGATAACCATTCCCGATAATGCAATTATGGTACTGCAACAGCAA
GATTGGCCACGTGGATGGCGAGATGACCCCACCCCCGTGGCCACCATGGACATCGGCACCGAATGGATTGGTTCAGTATC
ATCTGTCGGTCTGCTGGTACCATCCACGTTAGTCCCTTTGGAAAGAAATATGCTGGTGAATCCTAAACACCGCGATTTCG
CCTCGTGCCTGAAAAGCATCAATCCACTGACGTTCAGTTTTGATCCTCGTCTAAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179161 WP_002887278.1 NZ_CP063881:c1770439-1770173 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179161 NZ_CP084635:1954700-1955146 [Yersinia ruckeri]
ATGAGAACTTATCACCCAAGCCCGATGGCCAAAGTCAGCGCACTATGGCGTGAGATTGGTCTACCCGCCAGTCGTGGCAC
CGCTTTGGTGGACAGCATAAAACTGGGATTTACTGTGGATGTGATCGACACTATACATTTATGGGCTGATATGCCTAAAT
CTGAAATATTACGCGCTACGGGCATTCCGAGCCGCAGTCTGACGCGCCGTCGTACCCATGATGGGCGTTTTACCCCGGAA
GAAAGCGAACGTATTGCACGGTTTGTCCGGGTTATGGATGCGGCGGTAGATTTGTTCGGTGGTGACCGCACCAAAGCAAC
CGCATGGATGTCGATGCCGGTCAAAGGGCTGGGTAATCGAACGCCCGATTCACTGCTGGAAACCGAAACCGGTGCGTTGG
AAATCTGCGACCTGATTGGTCGTCTCGAACATGGGGTCTTTTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References