Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30301..30944 Replicon plasmid pM169-3.2
Accession NZ_CP063880
Organism Klebsiella pneumoniae strain M169-3

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag ISB47_RS27925 Protein ID WP_001044770.1
Coordinates 30301..30717 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag ISB47_RS27930 Protein ID WP_001261282.1
Coordinates 30714..30944 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB47_RS27900 26502..27146 + 645 WP_014386481.1 quinolone resistance pentapeptide repeat protein QnrB1 -
ISB47_RS27905 27238..28146 - 909 WP_001749993.1 SDR family oxidoreductase -
ISB47_RS27910 28495..28851 + 357 WP_072204799.1 DUF4158 domain-containing protein -
ISB47_RS27920 29538..30227 - 690 Protein_35 AAA family ATPase -
ISB47_RS27925 30301..30717 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
ISB47_RS27930 30714..30944 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
ISB47_RS27935 30901..31128 + 228 Protein_38 hypothetical protein -
ISB47_RS27940 31455..31994 - 540 WP_227543088.1 hypothetical protein -
ISB47_RS27945 32715..33065 + 351 WP_000493378.1 hypothetical protein -
ISB47_RS27950 33116..33859 + 744 WP_000129823.1 hypothetical protein -
ISB47_RS27955 33856..34632 + 777 WP_000015958.1 site-specific integrase -
ISB47_RS27960 34690..34947 - 258 WP_000764642.1 hypothetical protein -
ISB47_RS27965 35076..35180 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA2 / dfrA14 / ARR-3 / ere(A) / ant(3'')-Ia / cmlA1 / qacE / sul1 / qnrB1 - 1..37458 37458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T179158 WP_001044770.1 NZ_CP063880:c30717-30301 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T179158 NZ_CP084625:1501504-1501818 [Pseudomonas lalkuanensis]
ATGATCTTTATCGAGACTCCGATCTTTACCAAGCGTCTGCGTGAGTTGCTCAGCGACGACAGTTATGCGGCGTTTCAGCG
CCAGTTGGCCGAGCGGCCGGATATGGGCGACGTGATCGAAGGCACCGGAGGCATTCGCAAAGTGCGTGTCGCCTCGGGTG
GACACGGTAAGCGGGGCGGGTCCAGGGTCATCTACTACCACTTCACTTCGGCTTCGCAGATTGCACTGCTGCTGATCTAC
CCGAAGAACGAGAAGGATGATCTGTCGGCCGATGAGCGAAAGGTGCTCAAGCAAATCATTGAGCGGTGGAGGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT179158 WP_001261282.1 NZ_CP063880:c30944-30714 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT179158 NZ_CP084625:1501824-1502111 [Pseudomonas lalkuanensis]
ATGAGCAAATTCTTTGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATCCACCGTGGCGAGCGCAAGCCTTCACG
GGAGTTCACCGTGGACGCGCTGCAGGTGAAGGAAATCCGCAAGGCCACCGGCCTGACCCAGGCCAAGTTTGCGGCCATGA
TCGATGTGCAGCTCGGCACATTGCGCAACTGGGAACAAGGTCGGCGCGAACCCACTGGCCCGGCCAAGGCGTTGCTGCGG
GCCATTCACAACGACCCCAAGCACGTTATCCAGGCACTGTCTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References