Detailed information of TA system TA179146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3206193..3206783
• Replicon: chromosome
• Accession: NZ_CP063878
• Organism: Klebsiella pneumoniae strain M169-3

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ISB47_RS15770
• Protein ID: WP_023341911.1
• Coordinates: 3206451..3206783 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: ISB47_RS15765
• Protein ID: WP_000288812.1
• Coordinates: 3206193..3206450 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB47_RS15735 (3202105)	3202105..3202179	+	75	Protein_3069	type II toxin-antitoxin system PemK/MazF family toxin	-
ISB47_RS15740 (3202530)	3202530..3203009	+	480	WP_228710964.1	hypothetical protein	-
ISB47_RS15745 (3203122)	3203122..3204276	+	1155	WP_237085555.1	hypothetical protein	-
ISB47_RS15750 (3204330)	3204330..3204911	+	582	WP_099184326.1	hypothetical protein	-
ISB47_RS15755 (3205194)	3205194..3205655	+	462	WP_004213450.1	hypothetical protein	-
ISB47_RS15760 (3205652)	3205652..3205858	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
ISB47_RS15765 (3206193)	3206193..3206450	+	258	WP_000288812.1	antitoxin	Antitoxin
ISB47_RS15770 (3206451)	3206451..3206783	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISB47_RS15780 (3207105)	3207105..3208541	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
ISB47_RS15790 (3208907)	3208907..3210361	-	1455	WP_004148975.1	AMP nucleosidase	-
ISB47_RS15795 (3210491)	3210491..3210736	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T179146 WP_023341911.1 NZ_CP063878:3206451-3206783 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T179146 NZ_CP084592:c2024064-2023807 [Mycobacterium intracellulare subsp. chimaera]
ATGAACATCGTCTTCGCGCCTCAAGCGTGGGACGACTACCTGCACTGGCAGACGGCCGACCGGGCCGTGCTCAAGCGGAT
CAACAAGCTCATCGCGGAGGCTTGCCGGTCGCCGCAGGCGGGCATCGGCAAGCCTGAGCCCCTGAAATACGGGCTCGTCG
GCGCCTGGTCCCGGCGAATTACCCAGGAACACCGGCTCGTCTATCGGGTCGTCGGCGATGACCTGCAGATCCTCCAGGCC
CGGTTTCACTACATCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT179146 WP_000288812.1 NZ_CP063878:3206193-3206450 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT179146 NZ_CP084592:c2024306-2024061 [Mycobacterium intracellulare subsp. chimaera]
GTGGCCATCACGGCGAGCGAGGCGCGCAAGAACCTGTTTCCCCTGATCGAGAAGGTGAACGCCGACCGGACGTCGATCGA
GATCACGTCGAAAGCTGGTCGCGCGGTGCTGATGTCCGCCGATGACTGGGAGGCGTGGCAGGAGACGGCATACCTGTTCC
AGTCCCCGGCGAACGCCCGCCGGCTCCTCGACGCCGCTGACGCCCTGGACAGGGGCCGCGGAATCCGGGCCGACCTCGAC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0