Detailed information of TA system TA179144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1751311..1751897
• Replicon: chromosome
• Accession: NZ_CP063878
• Organism: Klebsiella pneumoniae strain M169-3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ISB47_RS08575
• Protein ID: WP_002920800.1
• Coordinates: 1751529..1751897 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: ISB47_RS08570
• Protein ID: WP_004174006.1
• Coordinates: 1751311..1751532 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB47_RS08550 (1747468)	1747468..1748394	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ISB47_RS08555 (1748391)	1748391..1749668	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
ISB47_RS08560 (1749665)	1749665..1750432	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISB47_RS08565 (1750434)	1750434..1751147	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISB47_RS08570 (1751311)	1751311..1751532	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISB47_RS08575 (1751529)	1751529..1751897	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISB47_RS08580 (1752170)	1752170..1753486	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISB47_RS08585 (1753593)	1753593..1754480	+	888	WP_032418302.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISB47_RS08590 (1754477)	1754477..1755322	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISB47_RS08595 (1755324)	1755324..1756394	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1746297..1759919	13622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T179144 WP_002920800.1 NZ_CP063878:1751529-1751897 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179144 NZ_CP084592:1600259-1600504 [Mycobacterium intracellulare subsp. chimaera]
ATGACGGCAGCTGCGAAGAGGGCGCTCACTCATTTGTTGCCCGAGTCGGTGGCCATTGCTTGCTGGGAGTTCATTCGGGG
TCCGCTCGCGGAGAACCCGCAGCGGGTGGGCAAGCCGCTTCGCGGCCAGTTGGAAGGGCGATACTCGGCGCGCAGGGGCG
AGTTTCGCGTCATTTACCGGATCTTCGACGAACGGGTGGTGGTCCCCGTCATCCACATCGCGCATCGCCGCGACGTTTAC
CGGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT179144 WP_004174006.1 NZ_CP063878:1751311-1751532 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179144 NZ_CP084592:1599960-1600244 [Mycobacterium intracellulare subsp. chimaera]
ATGTCGACGCTTCCGCTGGCCGAGGTCCGAGCCAACCTTTCCAAGCTGGTCGACGAGGCCGTCCGGACCCATGAGCGCAT
CGAGGTCACCAGGCAAGGTCGCCGCGCGGCGGTGATTCTCAGCGCGGATGACTTCGATTCGATTATGGAAACTCTGGCCA
TCCTCAGCGATCAAGACTTGATGCGCGAAGTCCGAGCGGCAGAGTCAGAGGCAGAGGACGGCGCGCTCTACACACTCGAT
CAGGTGACCGAGGAGATGCGTGCTGTCGGCCGGCTCCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7