Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1350601..1351226 Replicon chromosome
Accession NZ_CP063878
Organism Klebsiella pneumoniae strain M169-3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISB47_RS06720 Protein ID WP_002882817.1
Coordinates 1350601..1350984 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISB47_RS06725 Protein ID WP_004150355.1
Coordinates 1350984..1351226 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB47_RS06705 (1347967) 1347967..1348869 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISB47_RS06710 (1348866) 1348866..1349501 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISB47_RS06715 (1349498) 1349498..1350427 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISB47_RS06720 (1350601) 1350601..1350984 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISB47_RS06725 (1350984) 1350984..1351226 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
ISB47_RS06730 (1351431) 1351431..1352348 + 918 WP_032418237.1 alpha/beta hydrolase -
ISB47_RS06735 (1352362) 1352362..1353303 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISB47_RS06740 (1353348) 1353348..1353785 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
ISB47_RS06745 (1353782) 1353782..1354642 - 861 WP_004146232.1 virulence factor BrkB family protein -
ISB47_RS06750 (1354636) 1354636..1355235 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179143 WP_002882817.1 NZ_CP063878:c1350984-1350601 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179143 NZ_CP084592:1358202-1358663 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACTATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179143 WP_004150355.1 NZ_CP063878:c1351226-1350984 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179143 NZ_CP084592:1358010-1358198 [Mycobacterium intracellulare subsp. chimaera]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCGGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References