Detailed information of TA system TA179142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1032662..1034343
• Replicon: chromosome
• Accession: NZ_CP063878
• Organism: Klebsiella pneumoniae strain M169-3

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: ISB47_RS05245
• Protein ID: WP_004146663.1
• Coordinates: 1032662..1033579 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISB47_RS05250
• Protein ID: WP_032418188.1
• Coordinates: 1033585..1034343 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB47_RS05225 (1027968)	1027968..1029488	+	1521	WP_032418186.1	sugar ABC transporter ATP-binding protein	-
ISB47_RS05230 (1029485)	1029485..1030483	+	999	WP_002885172.1	ribose ABC transporter permease	-
ISB47_RS05235 (1030514)	1030514..1031455	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
ISB47_RS05240 (1031530)	1031530..1032537	+	1008	WP_032418187.1	carbohydrate kinase family protein	-
ISB47_RS05245 (1032662)	1032662..1033579	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISB47_RS05250 (1033585)	1033585..1034343	-	759	WP_032418188.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISB47_RS05255 (1034429)	1034429..1035157	-	729	WP_004210017.1	response regulator transcription factor	-
ISB47_RS05260 (1035430)	1035430..1037577	+	2148	WP_077598926.1	formate dehydrogenase subunit alpha	-
ISB47_RS05265 (1037574)	1037574..1037717	-	144	WP_022631365.1	hypothetical protein	-
ISB47_RS05270 (1037923)	1037923..1038609	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T179142 WP_004146663.1 NZ_CP063878:c1033579-1032662 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179142 NZ_CP084592:c1137105-1136698 [Mycobacterium intracellulare subsp. chimaera]
GTGATCTACCTGGATACCTCGGCTCTGGTCAAGCTGATCCGGATCGAAGTCGAATCTGATGCTCTAGCTGACTGGCTCGA
TGAACGCACCGAAACACGTTGGATAACATCTGCCTTGGCTGAAGTGGAGCTCCCCAGAGCCATCCGCGCAGTCGCCCCAA
ATGGCTTATCTGCTGTCCCCTCTGTGCTGGCCAGACTGGATCGGTTCGAAATCGACCAGGTCATCCGCGCCACCGCGGCC
GCCTACCCCGATCCGGCGCTGCGCTCGCTCGATGCGATACACCTGGCCACCGCTCAAATCGCCGCATCCACAGCACCATT
GACCGCCATGGTCGCCTATGACAGTCGACTCAGCGACGCCGCCCGTGCCCTCGGGATAACGATTGTCACACCTGGAAGAA
ACTCATGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28620.99 Da
• Isoelectric Point: 10.0001

>AT179142 WP_032418188.1 NZ_CP063878:c1034343-1033585 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPETLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179142 NZ_CP084592:c1137381-1137109 [Mycobacterium intracellulare subsp. chimaera]
ATGAGTGAAGTACCTCTCCGAATGCTCAACCAAGAGACGGCAAAGGTCTTGGCCCGCGTCAAGCGTGGCGAAGAGATCAC
GCTTACTGAGCGCGGGGAAGTCATCGCCCGAATAATCCCCGCTACCGCCGGCCCACTGGATGCGTTGATCACTGCCGGCA
GAGTGCAACCGGCGACGCTGGCCGGCCCAGCCCCGCGGCCGACCGTTCCGATGCACGACAATCTCGACGCCGGCGCGCTA
TTGCAACGTCTGCGCGCCGAGGAAAGGTATTAG

Structures

Toxin

• AlphaFold DB: J2XCI1