Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 751760..752570 Replicon chromosome
Accession NZ_CP063878
Organism Klebsiella pneumoniae strain M169-3

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISB47_RS03890 Protein ID WP_004178461.1
Coordinates 751760..752293 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISB47_RS03895 Protein ID WP_002887278.1
Coordinates 752304..752570 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB47_RS03885 (750591) 750591..751712 + 1122 WP_004214138.1 cupin domain-containing protein -
ISB47_RS03890 (751760) 751760..752293 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
ISB47_RS03895 (752304) 752304..752570 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
ISB47_RS03900 (752673) 752673..754106 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
ISB47_RS03905 (754096) 754096..754779 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISB47_RS03910 (754951) 754951..756336 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
ISB47_RS03915 (756354) 756354..756698 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179141 WP_004178461.1 NZ_CP063878:c752293-751760 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179141 NZ_CP084592:1072019-1072411 [Mycobacterium intracellulare subsp. chimaera]
ATGGCGGCCATCTACCTGGACTCGTCCGCAATCGTCAAGCTTGCAGTTCGTGAGCCAGAGTCGGATGCGCTGCGTCGCTA
CCTTCGCACCCGGCTGCCGCGGGTGTCGAGTGCCCTTGCGCGGACGGAGGTCATGCGGGCTCTGCTCCACAAGGGTGAAT
CAGCCCGAAGAGCCGGACGCCGGGCGCTCGCGAGCCTAGACCTACTACGCATCGACAACCGATTGCTCGATCTTGCTGGT
GGGCTGCTGCCAATCGAGCTACGCACCCTCGATGCAATTCACCTGGCGACTGCCCAGCGGCTCGGCATGGATCTTGGCCG
GTTGTGCACTTACGACGACCGCATGCGCGACGCCGCCGAGGCGCTCGGCATGGCAGTGATTGCCCCGTCTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179141 WP_002887278.1 NZ_CP063878:c752570-752304 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179141 NZ_CP084592:1071744-1072019 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTACGGTGGGCGTTCGTGAGCTGCGGCAGCGGGCCAGCGAACTCCTCCGTCGGGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGTGGTCGGCCTATCGCCCTTCTCTCGCCGATGCCGGAGGGCGGTCCGTACCAGCAGATGCTGGCAAGCG
GCGGGATCGAACGTGCGACGCTTGACTTTGATGACCTTCCCGAGCCGGTCCGACTCAAAACGGGTATCGAGCTGCCGTCC
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References