Detailed information of TA system TA179135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 12296..12939
• Replicon: plasmid pS174-1.2
• Accession: NZ_CP063876
• Organism: Klebsiella quasipneumoniae strain S174-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IOD26_RS26775
• Protein ID: WP_016236302.1
• Coordinates: 12523..12939 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: IOD26_RS26770
• Protein ID: WP_001261282.1
• Coordinates: 12296..12526 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD26_RS26735	7821..8687	-	867	WP_004118283.1	replication initiation protein	-
IOD26_RS26740	9222..9326	+	105	WP_032409716.1	hypothetical protein	-
IOD26_RS26745	9455..9712	+	258	WP_000764642.1	hypothetical protein	-
IOD26_RS26750	9770..10549	-	780	WP_138827844.1	site-specific integrase	-
IOD26_RS26755	10546..11367	-	822	WP_004213562.1	hypothetical protein	-
IOD26_RS26760	11422..11721	-	300	WP_052433691.1	hypothetical protein	-
IOD26_RS26765	11926..12339	-	414	WP_200983667.1	hypothetical protein	-
IOD26_RS26770	12296..12526	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IOD26_RS26775	12523..12939	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
IOD26_RS26780	13013..13249	+	237	WP_001348075.1	AAA family ATPase	-
IOD26_RS26785	13296..14000	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IOD26_RS26790	14285..15130	+	846	WP_001749984.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA16	-
IOD26_RS26795	15247..15594	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
IOD26_RS26800	15588..16427	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA16 / qacE / sul1 / qnrB6 / aac(6')-Ib-cr / ARR-3 / dfrA27 / tet(D)	-	1..56886	56886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T179135 WP_016236302.1 NZ_CP063876:12523-12939 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T179135 NZ_CP084586:4437615-4437917 [Mycobacterium intracellulare subsp. chimaera]
ATGATCGCGCCGGGCGACATCGCGCCGCGGCGAGACACCCGCCGCGACCTCTACGTCGCGGTGCTCTCCAACGCGATCCA
CCTCGCGGCCAACACTGGTCGCGTCATCGTCTGCCCGTTCATCCCTGGGAAAATCCCCGACGACACGATGGCCATGGTCG
TTCCGCTCACGCAGCCCGAAGGCACTCTTCTCCCCGAACTGGTGCAGTGGCTGCCGACTTCGGCGCTCGGTGAACCGATC
GGGGCCGTGTCGGCGGCCGCGCTTAGCGAGGCGATGTCGATCGTGACCGCGCTGATCACGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT179135 WP_001261282.1 NZ_CP063876:12296-12526 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT179135 NZ_CP084586:4437391-4437618 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAAGAGAAGATTTCTGTCACCGTCGACGCCGCCGTGCTCGCCGACGCCGACTCCGACGCCAAAGCGGCCGGACT
GAATCGCTCCGAGATGATCGAGCGGGCGCTGCGCAATGAGCATCTACGTCGCGCGCTTCATACCTATACGACGCGGACCG
CACCAGCGCTCAACATCGACTCCTACGCCGGGCAAGTGTATGAAGCCAACCGGGCCGCGGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3