Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 121304..121829 Replicon plasmid pS141.1
Accession NZ_CP063872
Organism Klebsiella pneumoniae strain S141

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag IRJ19_RS26880 Protein ID WP_004197633.1
Coordinates 121304..121609 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag IRJ19_RS26885 Protein ID WP_015632547.1
Coordinates 121611..121829 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ19_RS26855 116886..118029 + 1144 Protein_126 IS3 family transposase -
IRJ19_RS26860 117992..118369 + 378 WP_158672279.1 DUF1173 family protein -
IRJ19_RS26865 118518..118823 + 306 WP_126034233.1 hypothetical protein -
IRJ19_RS26870 119145..119930 - 786 WP_048291886.1 site-specific integrase -
IRJ19_RS26875 120129..121117 - 989 Protein_130 hypothetical protein -
IRJ19_RS26880 121304..121609 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
IRJ19_RS26885 121611..121829 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IRJ19_RS26890 122108..123481 + 1374 WP_048265005.1 hypothetical protein -
IRJ19_RS26895 123823..124251 - 429 WP_023345594.1 nuclear transport factor 2 family protein -
IRJ19_RS26900 124364..125209 - 846 WP_023345593.1 SDR family oxidoreductase -
IRJ19_RS26905 125312..125707 + 396 WP_023345592.1 helix-turn-helix transcriptional regulator -
IRJ19_RS26910 125819..126403 + 585 WP_023345591.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ybtQ / ybtX / ybtS / fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP 1..194302 194302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T179116 WP_004197633.1 NZ_CP063872:c121609-121304 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179116 NZ_CP084543:108505-108855 [Klebsiella michiganensis]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT179116 WP_015632547.1 NZ_CP063872:c121829-121611 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT179116 NZ_CP084543:108860-109162 [Klebsiella michiganensis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References