Detailed information of TA system TA179113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 53411..54150
• Replicon: plasmid pS141.1
• Accession: NZ_CP063872
• Organism: Klebsiella pneumoniae strain S141

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IRJ19_RS26475
• Protein ID: WP_021312536.1
• Coordinates: 53411..53896 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IRJ19_RS26480
• Protein ID: WP_003026799.1
• Coordinates: 53884..54150 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ19_RS26445	48555..48992	-	438	WP_126034224.1	hypothetical protein	-
IRJ19_RS26450	49088..50155	-	1068	WP_048291875.1	hypothetical protein	-
IRJ19_RS26455	50224..50520	-	297	WP_048291874.1	hypothetical protein	-
IRJ19_RS26460	50754..51131	+	378	WP_032719541.1	hypothetical protein	-
IRJ19_RS26465	51328..52302	-	975	WP_048291873.1	hypothetical protein	-
IRJ19_RS26470	52907..53068	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
IRJ19_RS26475	53411..53896	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
IRJ19_RS26480	53884..54150	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IRJ19_RS26485	54283..54711	-	429	WP_004901287.1	GFA family protein	-
IRJ19_RS26490	54912..55058	-	147	WP_159193595.1	IS3 family transposase	-
IRJ19_RS26495	55080..56200	+	1121	WP_085955508.1	IS3 family transposase	-
IRJ19_RS26500	56227..57042	-	816	WP_077258365.1	IS3 family transposase	-
IRJ19_RS26505	56997..57506	-	510	WP_004901294.1	IS3 family transposase	-
IRJ19_RS26510	58202..58462	+	261	WP_048291871.1	hypothetical protein	-
IRJ19_RS26515	58573..59043	-	471	WP_004144067.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ybtQ / ybtX / ybtS / fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP	1..194302	194302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T179113 WP_021312536.1 NZ_CP063872:c53896-53411 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T179113 NZ_CP084542:c4880477-4880220 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCTTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179113 WP_003026799.1 NZ_CP063872:c54150-53884 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179113 NZ_CP084542:c4880209-4879658 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCAGGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66