Detailed information of TA system TA179102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4168132..4168722
• Replicon: chromosome
• Accession: NZ_CP063871
• Organism: Klebsiella pneumoniae strain S141

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: IRJ19_RS20095
• Protein ID: WP_023341911.1
• Coordinates: 4168390..4168722 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: IRJ19_RS20090
• Protein ID: WP_000288812.1
• Coordinates: 4168132..4168389 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ19_RS20065	4163958..4164200	-	243	WP_004899316.1	hypothetical protein	-
IRJ19_RS20070	4164467..4166214	+	1748	Protein_3908	hypothetical protein	-
IRJ19_RS20075	4166293..4166850	+	558	WP_087638062.1	hypothetical protein	-
IRJ19_RS20080	4167133..4167594	+	462	WP_200985705.1	hypothetical protein	-
IRJ19_RS20085	4167591..4167797	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
IRJ19_RS20090	4168132..4168389	+	258	WP_000288812.1	antitoxin	Antitoxin
IRJ19_RS20095	4168390..4168722	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IRJ19_RS20105	4169044..4170480	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
IRJ19_RS20115	4170846..4172300	-	1455	WP_004148975.1	AMP nucleosidase	-
IRJ19_RS20120	4172430..4172675	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T179102 WP_023341911.1 NZ_CP063871:4168390-4168722 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T179102 NZ_CP084532:c4488666-4488355 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT179102 WP_000288812.1 NZ_CP063871:4168132-4168389 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT179102 NZ_CP084532:c4488354-4488064 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0