Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2263016..2263641 Replicon chromosome
Accession NZ_CP063871
Organism Klebsiella pneumoniae strain S141

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IRJ19_RS10845 Protein ID WP_002882817.1
Coordinates 2263016..2263399 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IRJ19_RS10850 Protein ID WP_004150355.1
Coordinates 2263399..2263641 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ19_RS10830 2260382..2261284 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IRJ19_RS10835 2261281..2261916 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IRJ19_RS10840 2261913..2262842 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
IRJ19_RS10845 2263016..2263399 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IRJ19_RS10850 2263399..2263641 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IRJ19_RS10855 2263846..2264763 + 918 WP_021466676.1 alpha/beta hydrolase -
IRJ19_RS10860 2264777..2265718 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IRJ19_RS10865 2265763..2266200 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IRJ19_RS10870 2266197..2267057 - 861 WP_004146232.1 virulence factor BrkB family protein -
IRJ19_RS10875 2267051..2267650 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179098 WP_002882817.1 NZ_CP063871:c2263399-2263016 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179098 NZ_CP084532:c3987226-3986939 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179098 WP_004150355.1 NZ_CP063871:c2263641-2263399 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179098 NZ_CP084532:c3986952-3986650 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References