Detailed information of TA system TA179092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 106413..107152
• Replicon: plasmid pM142-3
• Accession: NZ_CP063868
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: ISD32_RS27550
• Protein ID: WP_013087172.1
• Coordinates: 106667..107152 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: ISD32_RS27545
• Protein ID: WP_012540086.1
• Coordinates: 106413..106679 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS27515	101644..102180	-	537	WP_013087166.1	GNAT family N-acetyltransferase	-
ISD32_RS27520	102305..104056	-	1752	WP_162875198.1	arsenical pump-driving ATPase	-
ISD32_RS27525	104083..104445	-	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
ISD32_RS27530	104521..105066	-	546	WP_047365773.1	sigma-70 family RNA polymerase sigma factor	-
ISD32_RS27535	105075..105788	-	714	WP_013087170.1	arsenical resistance protein ArsH	-
ISD32_RS27540	105790..106113	-	324	WP_065907728.1	metalloregulator ArsR/SmtB family transcription factor	-
ISD32_RS27545	106413..106679	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
ISD32_RS27550	106667..107152	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
ISD32_RS27555	107494..107652	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
ISD32_RS27560	108556..108936	+	381	WP_038990973.1	hypothetical protein	-
ISD32_RS27565	109003..109350	+	348	WP_038990975.1	hypothetical protein	-
ISD32_RS27570	109438..109668	+	231	WP_038990977.1	hypothetical protein	-
ISD32_RS27575	109715..110548	+	834	WP_040107920.1	type I restriction-modification system subunit M	-
ISD32_RS27580	111173..111703	+	531	WP_023292160.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..180701	180701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T179092 WP_013087172.1 NZ_CP063868:106667-107152 [Klebsiella variicola]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T179092 NZ_CP084532:836174-836572 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT179092 WP_012540086.1 NZ_CP063868:106413-106679 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179092 NZ_CP084532:835947-836174 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8