Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 62487..63130 Replicon plasmid pM142-3
Accession NZ_CP063868
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag ISD32_RS27310 Protein ID WP_032720618.1
Coordinates 62487..62903 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag ISD32_RS27315 Protein ID WP_032720617.1
Coordinates 62900..63130 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS27290 58383..58895 - 513 WP_032720621.1 GrpB family protein -
ISD32_RS27295 59262..59456 + 195 Protein_64 transposase -
ISD32_RS27300 59844..60866 - 1023 WP_032720620.1 hypothetical protein -
ISD32_RS27305 60851..62413 - 1563 WP_032720619.1 AAA family ATPase -
ISD32_RS27310 62487..62903 - 417 WP_032720618.1 type II toxin-antitoxin system VapC family toxin Toxin
ISD32_RS27315 62900..63130 - 231 WP_032720617.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ISD32_RS27320 63471..64337 + 867 WP_050548602.1 hypothetical protein -
ISD32_RS27325 64427..65668 - 1242 WP_032720616.1 RES family NAD+ phosphorylase -
ISD32_RS27330 65733..66317 - 585 WP_032720615.1 hypothetical protein -
ISD32_RS27335 66627..67559 - 933 WP_032720614.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..180701 180701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15179.60 Da        Isoelectric Point: 7.2453

>T179091 WP_032720618.1 NZ_CP063868:c62903-62487 [Klebsiella variicola]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T179091 NZ_CP084532:825270-825683 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 4.3775

>AT179091 WP_032720617.1 NZ_CP063868:c63130-62900 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT179091 NZ_CP084532:825023-825289 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References