Detailed information of TA system TA179090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 43351..43934
• Replicon: plasmid pM142-3
• Accession: NZ_CP063868
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ISD32_RS27220
• Protein ID: WP_200991791.1
• Coordinates: 43351..43692 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: ISD32_RS27225
• Protein ID: WP_044265857.1
• Coordinates: 43704..43934 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS27195	38680..39885	-	1206	WP_044265872.1	PatB family C-S lyase	-
ISD32_RS27200	39917..41224	-	1308	WP_044265869.1	amino acid permease	-
ISD32_RS27205	41433..41894	+	462	WP_044265866.1	Lrp/AsnC family transcriptional regulator	-
ISD32_RS27210	42270..42395	+	126	Protein_47	IS5/IS1182 family transposase	-
ISD32_RS27215	42402..43103	-	702	WP_044265986.1	aspartate/glutamate racemase family protein	-
ISD32_RS27220	43351..43692	-	342	WP_200991791.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISD32_RS27225	43704..43934	-	231	WP_044265857.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISD32_RS27230	44323..46461	+	2139	WP_044265854.1	AAA family ATPase	-
ISD32_RS27235	46741..47622	-	882	WP_044265851.1	hypothetical protein	-
ISD32_RS27240	47615..48223	-	609	WP_044265848.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..180701	180701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 11917.41 Da
• Isoelectric Point: 3.9615

>T179090 WP_200991791.1 NZ_CP063868:c43692-43351 [Klebsiella variicola]
MLDTRFCAFILREQPEAVLGWLEQAVLRGHRIVISAITWAELSQAAREDSPATQALADAFCASLDVVLPWDRAAVDATTE
IKAALAATGTPLGPNPDAAPASDETAAGDDIPF

Nucleotide

• Length: 342 bp

>T179090 NZ_CP084532:358943-359218 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8575.81 Da
• Isoelectric Point: 5.0957

>AT179090 WP_044265857.1 NZ_CP063868:c43934-43704 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDCIILRPVRPAWNSFALMGKAGPEFMAEREVVISDNGRGEQ

Nucleotide

• Length: 231 bp

>AT179090 NZ_CP084532:358680-358940 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA