Detailed information of TA system TA179087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4261047..4261633
• Replicon: chromosome
• Accession: NZ_CP063867
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: ISD32_RS20355
• Protein ID: WP_094955330.1
• Coordinates: 4261265..4261633 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: ISD32_RS20350
• Protein ID: WP_004174006.1
• Coordinates: 4261047..4261268 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS20330	4257204..4258130	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ISD32_RS20335	4258127..4259404	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ISD32_RS20340	4259401..4260168	+	768	WP_012967117.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISD32_RS20345	4260170..4260883	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISD32_RS20350	4261047..4261268	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISD32_RS20355	4261265..4261633	+	369	WP_094955330.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISD32_RS20360	4261925..4263241	+	1317	WP_008806974.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISD32_RS20365	4263348..4264235	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISD32_RS20370	4264232..4265077	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISD32_RS20375	4265079..4266149	+	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4258127..4266886	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13538.90 Da
• Isoelectric Point: 8.6410

>T179087 WP_094955330.1 NZ_CP063867:4261265-4261633 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMSVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179087 NZ_CP084530:999372-999722 [Paenibacillus sp. YPG26]
ATGATCGTGAAGCGCGGTGACGTGTTTTTTGCTGACCTCTCACCTGTTGTAGGTTCCGAGCAGGGAGGAGTCAGACCGGT
GCTGATTATCCAGAACGATATCGGGAACCGTTTCAGCCCAACGGCCATAGTTGCTGCGATTACGGCTCAAATTCAGAAGG
CGAAGCTGCCGACTCATGTTGAGATTGAAGCGGCTACGCACGGGTTTGACCGGGATTCCGTCATTCTACTGGAGCAGATA
CGAACCATTGACAAGCAAAGGCTGACTGACAAGATCACCCATCTGGATGAAGAGACGATGAAGAAGGTGGATGAGTCACT
GCTTATCAGTGTGGGACTGATTGATTTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT179087 WP_004174006.1 NZ_CP063867:4261047-4261268 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179087 NZ_CP084530:999088-999369 [Paenibacillus sp. YPG26]
TTGGCCAATATGCAGAGCACCAAAAGAATCATGATCAGCTTGCCAGATTATCTTTTGCAAGAGGTGGATGGGATTGTAGC
TCTGGAGAATTCCAACCGCAGTGAACTTATTAGGCAGGCAATGAAGCTCTACTTGAACGAGCGGAAGAAGCGTTATATCC
GTGAGTCCATGCAGCGCGGTTACATGGAGATGGCGAAGATTAACCTGACCATGGCCTCTGAAGCGTTCCATGCGGAGGAA
GATGCGGACAGCACTCTGGACCGGCTAGTAAGCGGGGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7