Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4220406..4221037 Replicon chromosome
Accession NZ_CP063867
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H4S6Z2
Locus tag ISD32_RS20145 Protein ID WP_008806943.1
Coordinates 4220406..4220681 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag ISD32_RS20150 Protein ID WP_004181489.1
Coordinates 4220678..4221037 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS20125 4216687..4217385 - 699 WP_004204935.1 dimethylmenaquinone methyltransferase -
ISD32_RS20130 4217396..4218349 - 954 WP_004204934.1 hydroxyacid dehydrogenase -
ISD32_RS20135 4218686..4218961 + 276 WP_004204933.1 LysR family transcriptional regulator -
ISD32_RS20140 4218996..4220009 - 1014 WP_008806942.1 magnesium transporter -
ISD32_RS20145 4220406..4220681 + 276 WP_008806943.1 type II toxin-antitoxin system HicA family toxin Toxin
ISD32_RS20150 4220678..4221037 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISD32_RS20155 4221065..4221466 - 402 WP_008806944.1 nickel-responsive transcriptional regulator NikR -
ISD32_RS20160 4221454..4222245 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
ISD32_RS20165 4222242..4223006 - 765 WP_023340796.1 nickel import ATP-binding protein NikD -
ISD32_RS20170 4223006..4223839 - 834 WP_008806947.1 nickel ABC transporter permease subunit NikC -
ISD32_RS20175 4223836..4224780 - 945 WP_110224117.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10295.87 Da        Isoelectric Point: 10.3900

>T179086 WP_008806943.1 NZ_CP063867:4220406-4220681 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVRP

Download         Length: 276 bp

>T179086 NZ_CP084529:4435367-4435654 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179086 WP_004181489.1 NZ_CP063867:4220678-4221037 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179086 NZ_CP084529:4435128-4435367 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4S6Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References