Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3849125..3849750 Replicon chromosome
Accession NZ_CP063867
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISD32_RS18465 Protein ID WP_008807903.1
Coordinates 3849125..3849508 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISD32_RS18470 Protein ID WP_004150355.1
Coordinates 3849508..3849750 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS18450 3846491..3847393 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISD32_RS18455 3847390..3848025 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISD32_RS18460 3848022..3848951 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISD32_RS18465 3849125..3849508 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISD32_RS18470 3849508..3849750 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISD32_RS18475 3849955..3850872 + 918 WP_064166397.1 alpha/beta hydrolase -
ISD32_RS18480 3850887..3851828 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
ISD32_RS18485 3851873..3852310 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISD32_RS18490 3852307..3853167 - 861 WP_008807907.1 virulence factor BrkB family protein -
ISD32_RS18495 3853161..3853760 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179085 WP_008807903.1 NZ_CP063867:c3849508-3849125 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179085 NZ_CP084529:4299684-4299962 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179085 WP_004150355.1 NZ_CP063867:c3849750-3849508 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179085 NZ_CP084529:4299421-4299681 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1F2M041


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References