Detailed information of TA system TA179084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3525840..3527521
• Replicon: chromosome
• Accession: NZ_CP063867
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: ISD32_RS16975
• Protein ID: WP_012543157.1
• Coordinates: 3525840..3526715 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A0B7GCX3
• Locus tag: ISD32_RS16980
• Protein ID: WP_008807257.1
• Coordinates: 3526763..3527521 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS16955	3521146..3522666	+	1521	WP_008807253.1	sugar ABC transporter ATP-binding protein	-
ISD32_RS16960	3522663..3523661	+	999	WP_012543154.1	ribose ABC transporter permease	-
ISD32_RS16965	3523692..3524633	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISD32_RS16970	3524708..3525715	+	1008	WP_023339423.1	carbohydrate kinase family protein	-
ISD32_RS16975	3525840..3526715	-	876	WP_012543157.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISD32_RS16980	3526763..3527521	-	759	WP_008807257.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISD32_RS16985	3527606..3528334	-	729	WP_110225030.1	response regulator transcription factor	-
ISD32_RS16990	3528607..3530754	+	2148	WP_077598880.1	formate dehydrogenase subunit alpha	-
ISD32_RS16995	3531460..3532146	+	687	WP_101855303.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 33088.17 Da
• Isoelectric Point: 5.4637

>T179084 WP_012543157.1 NZ_CP063867:c3526715-3525840 [Klebsiella variicola]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQAGMNAVLQANRSDE
MRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKSCFTQQDIDFLLSFK
RGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T179084 NZ_CP084529:c4293623-4293225 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28653.01 Da
• Isoelectric Point: 10.1988

>AT179084 WP_008807257.1 NZ_CP063867:c3527521-3526763 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179084 NZ_CP084529:c4293919-4293626 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B7GCX3