Detailed information of TA system TA179083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3359581..3360097
• Replicon: chromosome
• Accession: NZ_CP063867
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ISD32_RS16150
• Protein ID: WP_022065401.1
• Coordinates: 3359581..3359865 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: ISD32_RS16155
• Protein ID: WP_002886901.1
• Coordinates: 3359855..3360097 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS16135	3354684..3356339	+	1656	WP_200991560.1	alpha,alpha-phosphotrehalase	-
ISD32_RS16140	3356725..3358863	+	2139	WP_200991561.1	anaerobic ribonucleoside-triphosphate reductase	-
ISD32_RS16145	3359113..3359577	+	465	WP_110215844.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
ISD32_RS16150	3359581..3359865	-	285	WP_022065401.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISD32_RS16155	3359855..3360097	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ISD32_RS16160	3360175..3362085	-	1911	WP_032731805.1	BglG family transcription antiterminator	-
ISD32_RS16165	3362108..3363262	-	1155	WP_012969087.1	lactonase family protein	-
ISD32_RS16170	3363388..3364128	-	741	WP_008807137.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11128.97 Da
• Isoelectric Point: 10.2849

>T179083 WP_022065401.1 NZ_CP063867:c3359865-3359581 [Klebsiella variicola]
MTYELEFDPRAWREWQMPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T179083 NZ_CP084529:4283031-4283372 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT179083 WP_002886901.1 NZ_CP063867:c3360097-3359855 [Klebsiella variicola]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT179083 NZ_CP084529:4282693-4283010 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0